柴胡提取物对人肝癌细胞BEL-7402细胞内游离钙离子浓度和细胞内VCR蓄积的影响  

Effects of the extracts from Bupleurum Chinese DC on intracelluar free calcium concentration and vincristine accumulation in human hepatoma BEL-7402 cells

作　　者：韩晓红[1] 盖晓东[2] 薛延军[1] 陈淼[1] 

机构地区：[1]江苏大学医学院病理教研室,镇江212001 [2]北华大学医学院病理教研室,吉林132013

出　　处：《肿瘤》2006年第4期314-317,共4页Tumor

基　　金：吉林省教育厅科研基金项目(编号:吉教合字2006第26号)

摘　　要：目的:测定中药柴胡(BupleurumChineseDC,BCDC)提取物对人肝癌BEL-7402细胞内游离钙离子浓度([Ca2+]i)和长春新碱(Vincristine,VCR)浓度的影响,探索柴胡提取物逆转BEL-7402细胞多药耐药的机制。方法:以Fura2/AM为细胞内钙离子的荧光指示剂,用双波长荧光分光光度计分别测定不同条件下的BEL7402细胞内[Ca2+]i。高效液相色谱法测定BCDC作用于BEL-7402后细胞内VCR蓄积情况的变化。结果:柴胡提取物可使人肝癌细胞BEL-7402细胞内游离钙离子浓度下降(P<0.001),可使细胞内VCR浓度升高(P<0.01)。结论:柴胡提取物可以增加VCR在BEL7402细胞内的积聚浓度,部分逆转BEL7402细胞的多药耐药(multidrugresistance,MDR)。钙离子通道阻滞作用可能为柴胡提取物逆转BEL7402细胞多药耐药的机制之一。Objective： To explore the mechanism by which Bupleurum Chinese DC （BCDC） reverses the mutidrug resistance （MDR） of human hepatoma BEL-7402 ceils by measuring intraeellular concentrations of free calcium and vincristine （VCR）. Methods： Intracellular free calcium concentration （[Ca^2＋ ]i） in human hepatoma BEL-7402 cells of different groups was measured by double wavelength speetrofluorometer with Fura-2/AM as the fluorescence indicator. Cellular VCR concentration after BCDC treatment was examined by high performance liquid chromatography （HPLC）. Results：Cellular VCR concentration in BEL-7402 cells was increased after treatment with 390 μg/ mL BCDC and 5μg/mL verapamil （VRP） compared with control,respectively （P〈0.01 ）. VRP and 390μg/mL BCDC increased intraccllalar VCR from （57. 212±0.032） pg/μL up to （60. 145~0. 028） pg/μL and （59. 196~0. 045）pg/bd.,respectively. BCDC reduced [Ca^2＋]i in human hepatoma BEL-7402 cells（P〈0.001）. Condusion：BCDC increased intraeellular VCR concentration in BEL-7402 cells indicating that BCDC partly reversed MDR of BEL-7402 cells. Blockade of calcium channels may be one of the mechanisms by which BCDC reverses the MDR of human hepatoma BEL- 7402 cells.

关 键 词：柴胡提取物 治疗应用 细胞内游离钙 FURA-2/AM 高效液相色谱法 BEL-7402细胞 

分 类 号：R273.57[医药卫生—中西医结合]