玉米大斑病菌特异性毒素组分分离条件的优化研究  

Study on Optimum Conditions of Isolation and Purification of Specific Toxin Fractions Produced by Exserohilum turcicum

作　　者：王绍新[1] 董金皋[1] 

机构地区：[1]河北农业大学真菌毒素实验室,保定071001

出　　处：《微生物学通报》2006年第2期6-9,共4页Microbiology China

基　　金：国家教育部骨干教师计划资助项目;河北省自然科学基金项目(No.302318);河北农业大学9816科技重点项目

摘　　要：把从玉米大斑病菌的1号小种菌株(99-2)中提取的粗毒素进行硅胶TL层析,分离到Ⅰ(Rf0·06)、Ⅱ(Rf0·21)、Ⅲ(Rf0·45)、Ⅳ(Rf0·60)、Ⅴ(Rf0·75)5种组分,分别对此5组分进行生物测定,发现组分Ⅱ对带Ht1基因的玉米具有较强的特异性。经对组分Ⅱ进行HPLC的进一步纯化,又可得到3种组分Ⅱ-1、Ⅱ-2、Ⅱ-3,其中只有Ⅱ-3具有特异致病活性。对Ⅱ-1、Ⅱ-2、Ⅱ-3进行紫外-可见全波长扫描,发现只有Ⅱ-3在300nm处有吸收峰,Ⅱ-1和Ⅱ-2的峰形相似,可能为类似物。Five fractions, Ⅰ （ Rf0. 06 ） , Ⅱ （ Rf0. 21 ）, Ⅲ （ Rf0. 45 ）, Ⅳ （ Rf0. 60 ）, Ⅴ （ Rf0. 75 ） have been obtained after HT-toxin from race 1 of Exserohilum turcicum 99-2 isolated by TLC. In all of these fractions, only fractions II had specific toxicity to the corn leaves with Ht1 gene, Then fractions Ⅱ -1, Ⅱ -2, Ⅱ -3 were isolated from fractions H by HPLC purification, and the bioassay result showed only fraction Ⅱ -3 was toxigentic to corn leaves with Ht1 gene but non-toxigentic to corn leaves without Htl gene. Fractions Ⅱ -1, Ⅱ -2 and Ⅱ-3 were scanned by UV-Vis spectrophotometer. It was shown that the fractions Ⅱ-1 and Ⅱ -2 had analogous spectrum, and especially the fraction Ⅱ-3 had a special peak at 300nm.

关 键 词：玉米大斑病 真菌毒素 玉米 

分 类 号：S435.13[农业科学—农业昆虫与害虫防治]