香菇多糖的分子量测定  

Determination of Lentinus Edodes Polysaccharide Average Molecular Weight

作　　者：骆传环[1] 黄荣清[1] 肖炳坤[1] 梁晓东[1] 赵焱[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《科学技术与工程》2006年第8期1058-1059,共2页Science Technology and Engineering

基　　金：北京市自然科学基金资助(7042048)

摘　　要：对能够提高免疫功能的香菇多糖测定其平均分子量。用分析型超速离心机沉降法5次测定,求出香菇多糖溶液的沉降系数S,再换用扩散泵,5次测定,求出该溶液的扩散系数D。通过公式MW=RTS/[D(1-ρυ)]求出分子量。式中MW为重均分子量,R为气体常数,T为绝对温度,υ为偏微比容,ρ为溶剂密度,计算出香菇多糖的重均分子量为36000道尔顿。为新药申报中有关质量研究,质量标准制定等项目资料,提供必要的方法和数据。Molecular Weight of Lentinus Edodes Polysaccharide （LEP） which can improve immunity function was determined. Subsidence cofficient （S） of LEP solution was gaind through 5 times works on anatysis supervelocity centrifuge. After changing rotor into Diffusion pump Diffusion coefficient （D） was floated as similar operation. Its Weight Average molecular Weight（^-Mw） was calculated with formula ^-Mw=RTS/[D（ 1-ρv], （in it, R： gas constant. T：absolute temperature v：declination slight specific volume, ρ：solvent density）. So ^-Mw of LEP was 36 000 Dolton, The result is very useful for new drug application about qualitative research and qualitative standard constituting and so on.

关 键 词：香菇多糖 分子量 超速离心法 

分 类 号：S646.12[农业科学—蔬菜学]