异种角膜基质作为生物角膜载体的免疫学研究  

Immunologic study on xenogeneic corneal stroma as biological carrier for corneal reconstruction

作　　者：吴静[1] 郝念[2] 李姝燕[3] 徐锦堂[1] 王彦平[4] 

机构地区：[1]暨南大学医学院眼科研究室,广东省广州市510632 [2]华中科技大学同济医学院附属协和医院,湖北省武汉市430030 [3]广东省东莞市人民医院眼科,51170 [4]暨南大学医学院病生教研室,广东省广州市510632

出　　处：《眼科新进展》2006年第5期324-327,共4页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:39870855);广东省科技计划项目基金资助(编号:2KM05203S);暨南大学侨办重点学科基金资助(编号:51205004)

摘　　要：目的探讨异种(猪)角膜基质组织免疫原性,以评价其作为角膜重建载体材料的可行性。方法将新鲜、脱水2种猪角膜基质植片分别植入F344大鼠角膜基质层间,术后12d、90d抽取外周血,行抗CD25·FITC和抗CD4/CD8·PE双色免疫荧光标记,流式细胞仪测定分析。结果测得新鲜组、脱水组大鼠术后12d外周血T淋巴细胞表面抗原CD4+和CD25+、CD8+和CD25+双阳性表达率(1.53%±0.47%,2.13%±0.53%与0·57%±0.20%,0.67%±0.18%)及CD4+/CD8+比值(1.43±0.28,1.69±0.18)与同基因移植组(1.78%±0.36%,1.50±0.19)、阴性对照组(2.06%±0.52%,1.44±0.32)比较无显著性差异(P>0.05)。术后90d,新鲜组、脱水组大鼠外周血T淋巴细胞表面抗原CD4+和CD25+、CD8+和CD25+双阳性表达率(1.48%±0.39%,1.50%±0.46%与0·55%±0.15%,0·58%±0·11%)及CD4+/CD8+比值(1.43±0.51,1.36±0.59)与同基因移植组(1.32%±0·75%,1.31±0.29)、阴性对照组(1.34%±0.18%,1.44±0.42)比较,也无显著性差异(P>0.05)。结论异种(猪)角膜基质免疫原性低,是一种理想的角膜体外重建载体材料。Objective To investigate the immunogenicity of xenogeneic swine corneal stroma as biological carrier for corneal reconstruction. Methods The lymphocytes from the peripheral blood of F344 rats were immunologically labeled by anti- CD25· FITC and anti- CD4/CD8 ·PE then determined by flow cytometry at 12 days and 90 days after intracorneal implantation with fresh and dehydrated swine corneal stroma. Results The expression of CD4^＋ and CD25^＋,CD8^＋ and CD25^＋ and ratio of CD4^＋/CD8^＋ were 1.53% ± 0.47% and 2.13%±0.53% ,0.57% ±0.20% and 0.67% ± 0.18%,1.43 ±0.28 and 1.69 ±0.18 respectively in fresh and dehydrated swine corneal stroma group at 12 days after intracorneal implantation, which were 1.48% ± 0.39% and 1.50% ± 0.46%, 0.55 % ± 0.15 % and 0.58 % ± 0.11% , 1.43 ± 0.51 and 1.36 ± 0.59 respectively at 90 days. Compared to isograft group and negative control group, xenograft（swine corneal stroma） groups＇ expression of CD4^＋ and CD25^＋ ,CD8^＋ and CD25^＋ and ratio of CD4^ ＋/CD8^＋ did not show significantly difference in statistics （P 〉 0.05） either at 12 days, or at 90 days after intracorneal implantation. Conclusion Swine corneal stroma has low immunogenicity and is an ideal biological carrier for cornea reconstruction in vitro.

关 键 词：角膜基质 免疫原性 角膜重建 生物载体 异种移植 

分 类 号：R779.65[医药卫生—眼科]