佛波酯等3种促癌物诱导BALB/c3T3细胞骨桥素基因表达升高  

Up-regulation of osteopontin gene in BALB/c 3T3 cells induced by tumor promoters of TPA,okadaic acid and cadmium chloride

作　　者：敖琳[1] 刘晋祎[1] 高利宏[1] 刘胜学[1] 杨梦苏[2] 曾志雄[2] 方志俊[2] 曹佳[1] 

机构地区：[1]第三军医大学军事预防医学院卫生毒理学教研室,重庆400038 [2]香港城市大学基因组科技应用研究中心

出　　处：《第三军医大学学报》2006年第9期876-879,共4页Journal of Third Military Medical University

基　　金：国家重点基础研究发展规划资助项目("973"项目)(2002CB512901);国家杰出青年基金资助项目(30125037);国家自然科学基金资助项目(30271136)~~

摘　　要：目的探讨促癌物佛波酯(12-O-tetradecanoylphorbol-13-acetate,TPA),岗田酸(okadaic ac id,OA)和氯化镉(cadm ium chloride,CdC l2)在促进BALB/c 3T3细胞转化过程中对骨桥素基因(OPN)转录表达的影响。方法以N-甲基-N′-硝基-N-亚硝基胍(MNNG)为启动剂,TPA,OA和CdC l2分别为促癌剂,建立BALB/c 3T3细胞转化模型。采用小鼠毒理基因芯片分别检测促癌物处理过程中和转化细胞集落中的基因表达变化,同时采用实时RT-PCR验证部分细胞样本的OPN基因表达。结果基因芯片检测结果显示,3种促癌物在作用过程中均能诱导OPN基因表达升高,其中TPA处理后4 h,7 d和17 d,OA处理后3,7 d和24 d,CdC l2处理后3 d和7 d,OPN基因的表达较对照组增强;同时在促癌物诱导形成的9个转化细胞集落中,有8个转化集落检测到OPN基因表达升高。此外,实时RT-PCR检测也验证了OPN基因的表达升高。结论OPN基因与TPA,OA和CdC l2的促癌作用存在密切联系。Objective To explore the changes of osteopontin （OPN） gene expression induced by tumor promoter of 12-O-tetradecanoylphorbol-13-acetate （ TPA）, okadaic acid （OA） or cadmium chloride （ CdC12 ）. Methods The two-stage transformation test of BALB/c 3T3 cells was established with MNNG as initiator, TPA, OA or CdC12 as promoter respectively. Mouse toxicology gene chip was used to detect the gene expression of BALB/c 3T3 cells transformed with TPA, OA or CdCl2 respectively, and the expression of OPN gene was validated by real-time RT-PCR assay. Results TPA, OA or CdCl2 could increase the transcriptional expression of OPN gene in BALB/c 3T3 cells. The expression of OPN gene was up-regulated in colonies induced by TPA, OA or CdC12 respectively. Moreover, the up-regulation of confirmed by real-time RT-PCR assay. Conclusion The up-regulation of OPN gene the eight transformed cell OPN gene expression was expression is closely relat- ed to cell transformation of BALB/c 3T3 cells induced by TPA, OA or CdCl2.

关 键 词：骨桥素基因 促癌物 佛波酯 岗田酸 氯化镉 

分 类 号：R394-33[医药卫生—医学遗传学] R730.231[医药卫生—基础医学]