犬可复性胆总管不全梗阻对Oddi括约肌功能的影响  

Effect of reversible partial obstruction of common bile duct on function of sphincter of Oddi in dogs

作　　者：刘永康[1] 禹建峰[1] 李智华[1] 徐世荣[2] 

机构地区：[1]第三军医大学西南医院全军肝胆外科研究所中国人民解放军肝脏移植重点实验室,重庆430038 [2]重庆大学生物力学与组织工程教育部重点实验室,重庆400044

出　　处：《第三军医大学学报》2006年第9期952-954,共3页Journal of Third Military Medical University

基　　金：高等学校重点实验室访问学者基金资助项目(重庆大学校科字[2003]76)~~

摘　　要：目的建立可复性胆总管不全梗阻的动物模型,研究胆总管不全梗阻对Odd i括约肌(sph incter of Odd i,SO)运动的影响。方法将成年杂种犬麻醉后,暴露并游离胆总管中段,不全梗阻(PO)组以丝线将胆总管和一直径1 mm的导管一同结扎后拔除导管,正常对照(NC)组不结扎胆总管。术后不同时间点获取血液和肝脏的标本分别检测肝功及肝脏的病理变化;术后第28天进行SO测压。结果术后28 d实验组胆总管梗阻完全解除,肝细胞形态结构恢复,肝功能正常;实验组SO基础压和峰压降低,收缩频率减慢,但收缩间期无明显变化。结论胆总管不全梗阻对SO运动具有抑制作用,该动物模型能够模拟短期的梗阻性黄疸对胆道动力学的影响,方法简便、易行,可重复性好。Objective To establish a reversible partial in dogs and explore the influence of partial obstruction Methods Adult mongrel dogs were anesthetized. In the obstruction model of the common bile duct （CBD） CBD on the motility of sphincter of Oddi （SO）. partial obstruction （PO） group, a double ligature was performed around both a cannula （1 mm in diameter） and the common bile duct near to its bifurcation, then the cannula was withdrawn, while in the control group, only sham operation was done. The serum of venous blood was obtained before and on day 1,3, 5, 7, 14, 28 after operation. Liver specimens were obtained by biopsy on postoperative day 3, 7, 14. SO motility was recorded on postoperative day 28. Results All the obsrtuction of CBDs were removed in PO group, the structure of hepatocytes and liver function recovered to normal on day 28 after traction （SOF） operation. SO basal pressure （ SOBP）, SO amplitude （SOAP） and frequency of SO phasic consignificantly decreased, whereas the duration of SO contraction did not change. Conclusion Partial obstruction of CBD inhibited the motility of SO. The clinical manifestation of this model is similar to that of patients with temporary obstructive jaundice that influenced the motility of SO. This model was relatively simple, easy and reliable.

关 键 词：胆总管 不全梗阻 ODDI括约肌 动物模型 

分 类 号：R-332[医药卫生] R322.48