膜受体介导的细胞凋亡与细胞周期的关系  

Correlation of Receptor-mediated Apoptosis to Cell Cycle

作　　者：杨长永[1] 谢大兴[1] 周毅[1] 黄丹[1] 龚建平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院肿瘤研究所/普外科,湖北武汉430030

出　　处：《癌症》2006年第5期576-581,共6页Chinese Journal of Cancer

基　　金：卫生部临床重点学科资助项目(No.20012537)~~

摘　　要：背景与目的:线粒体介导的细胞凋亡大部分与细胞周期相关,但膜受体介导的细胞凋亡是否与细胞周期有关尚不清楚。本研究旨在观察膜受体介导的人类细胞凋亡与细胞周期特异性,阐明膜受体介导的细胞凋亡与细胞周期的关系。方法:用肿瘤坏死因子-α、抗Fas抗体分别处理指数生长期的Molt-4、Jurkat以及健康人外周血淋巴细胞;应用AnnexinV/PI和API等方法,通过流式细胞仪检测细胞凋亡和细胞周期特异性。结果:处于静止期的外周血淋巴细胞对凋亡诱导剂不敏感,凋亡率是6%～8%。Molt-4、Jurkat细胞以及加入植物血凝素刺激的外周血淋巴细胞经肿瘤坏死因子-α、抗Fas抗体诱导后出现了明显的细胞凋亡,凋亡率是15%～28%。膜受体介导的细胞凋亡主要发生在细胞周期的早G1期。结论:膜受体介导的细胞凋亡与细胞周期相关,具有细胞周期特异性。BACKGROUND ＆ OBJECTIVE： Chondriosome-mediated apoptosis is closely related to cell cycle, however, the correlation of receptor-mediated apoptosis to cell cycle progression is unclear yet. This study was to observe the receptor-mediated apoptosis and cell cycle specificity in cultured normal and tumor lymphocytes, and investigate their correlation. METHODS： Exponentially growing human leukemia cell lines Molt- 4 and Jurkat were treated with tumor necrosis factor-α （TNF-α） or Anti-Fas. Peripheral blood lymphocytes （PBLs） from healthy donors were stimulated by phytohemagglutinin （PHA）, and further incubated with the presence of TNF-α or anti-Fas. Annexin V/PI was used to detect the apoptosis, and API method was used to illustrate the cell cycle specificity of apoptotic cells. RESULTS： Unstimulated PBLs kept blunt to stimulation with TNF-α or anti-Fas, and the apoptotic rate was 6%-8%. Molt-4 cells, Jurkat cells, and stimulated PBLs which were treated with TNF-α or anti-Fas went to apoptosis, and the apoptosis rates were 15%-28%. Most receptor-mediated apoptosis happened in early G1 phase. CONCLUSION： Receptor-mediated apoptosis is closely related to cell cycle and presents cell cycle specificity.

关 键 词：膜受体 细胞周期 凋亡 白血病 Molt-4细胞株 JURKAT细胞株 外周血淋巴细胞 增殖 

分 类 号：R73-3[医药卫生—肿瘤]