促血管生成素及其受体的异常表达与胃癌血管生成的关系  

Correlation between the expression of angiopoietins and their receptor and angiogenesis in gastric cancers

作　　者：张真真[1] 张声[1] 林建银[2] 黄培生[3] 陈余鹏 

机构地区：[1]福建医科大学附属第一医院病理科,福州350005 [2]福建医科大学分子医学教研室 [3]福建医科大学病理学系

出　　处：《中华肿瘤杂志》2006年第4期280-284,共5页Chinese Journal of Oncology

基　　金：福建省自然科学基金资助项目(C0110013)

摘　　要：目的探讨促血管生成素(Ang)及其受体Tie-2在胃癌中的表达及其与胃癌血管生成的关系。方法应用RT-PCR和免疫组化分析技术,检测68例胃癌组织及其相应的癌旁胃黏膜Ang-1、Ang-2及Tie-2mRNA和蛋白表达水平,计数微血管密度(MVD)。结果胃癌组织及相应癌旁组织均见Ang-1、Ang-2及Tie-2mRNA的表达,Ang-1蛋白、Tie-2mRNA表达水平与MVD呈负相关(r=-0.440,r=-0.267;P<0.05),Ang-2mRNA及蛋白表达水平与MVD呈正相关(r=0.319,r=0.729;P<0.05),Ang-2/Ang-1蛋白表达的比值与MVD呈正相关(r=0.739,P<0.05)。在Ang-2mRNAT/N比值(胃癌与癌旁胃粘膜mRNA表达水平的比值)>1.2时,其MVD均高于T/N比值<1.2者,而与Ang-1mRNA的表达情况无关。结论Ang-1与Ang-2蛋白在胃癌血管生成中相互拮抗,Ang-2/Ang-1的比值可能是决定胃癌血管生成和肿瘤生长的最终因素。当Ang-2高表达而Ang-1低表达时,促进胃癌的血管生成;反之,则抑制胃癌血管生成。推测Angs及其受体系统在胃癌血管生成中的调节作用是以Ang-2的作用为主导的。Objective To explore the effects of angiopoietins （Ang-1 and Ang-2） and Tie-2 expression on microvessel density （MVD） in gastric cancers. Methods By using semiquantitative RT- PCR, immunohistochemistry and image analysis system, the expression of Ang-1, Ang-2, Tie-2 mRNA and their proteins were detected in 68 primary gastric cancers and their adjacent normal tissues. Microvessel density （MVD） was figured out based on CD34 immunohistochemical staining. Results The expression of all Ang-1, Ang -2, Tie-2 mRNA and their proteins was detected in gastric cancers and their paired adjacent gastric mucosa tissues. A negative correlation between Ang-1 protein, Tie-2 mRNA and MVD in gastric cancers was observed （ r = - 0.440, r = - 0. 267 ; P 〈 0.05 ）, while the relation between Ang-2 mRNA and its protein, Ang-2/Ang-1 protein ratio with MVD were positive （ r = 0.319 ,r =0. 729 ,r = 0.739 ;P 〈0.05 ）. It was found that MVD in groups with Ang-2 mRNA T/N ratio over 1.2 （ the ratio of Ang-2 mRNA in gastric cancers and its adjacent normal mucosa） was higher than that in those with a ratio under 1.2, revealed by analysing the effects of Ang-1 and Ang-2 mRNA T/N ratio on MVD in gastric cancers. Conclusion Ang-1 activates Tie-2 receptor, whereas Ang-2 antagonizes Ang-1 in the angiogenesis, and the Ang-2/Ang-1 ratio determines angiogenesis and tumor growth in gastric cancers. When the expression of Ang-2 is high and Ang- 1 is low, the angiogenesis in gastric cancers is promoted, otherwise oppositely. The role of Ang-2 is dominant in the effect of Angs and their receptor on angiogenesis in gastric cancers.

关 键 词：促血管生成素 TIE-2 胃肿瘤 

分 类 号：R735.2[医药卫生—肿瘤]