局部联合应用雷帕霉素及环孢霉素A在大鼠角膜移植排斥反应中的作用(英文)  

Effect of locally administered rapamycin and cyclosporin A on penetrating keratoplasty rejec- tion rat model

作　　者：李兵[1] 洪晶[2] 魏杰[1] 赵力艳[1] 

机构地区：[1]锦州医学院附属第一医院眼科,中国辽宁省锦州市121001 [2]中国医科大学附属第一医院眼科,中国辽宁省沈阳市110001

出　　处：《国际眼科杂志》2006年第2期271-274,共4页International Eye Science

摘　　要：目的:评价雷帕霉素滴眼液与环孢霉素A滴眼液联合应用对大鼠角膜移植免疫排斥反应的抑制作用。方法:建立大鼠穿透性角膜移植动物模型,以SD大鼠为受体,Wistar大鼠为供体,分为A,B,C,D4组。手术后2d术眼分别滴用滴眼液基质100μL,2g/L雷帕霉素滴眼液100μL,10g/L环孢霉素A滴眼液100μL,2g/L雷帕霉素滴眼液50μL+10g/L环孢霉素A滴眼液50μL,4次/d,连续用药至排斥反应发生。对角膜植片进行临床观察,记录植片存活时间,并进行组织学检查。结果:各治疗组显著延长角膜植片存活时间(P<0.01),联合用药的D组效果更佳,优于各单药物治疗组B组和C组(P<0.05,P<0.01)。组织学发现,术后14d各治疗组炎细胞浸润、新生血管形成及水肿程度明显轻于对照组。结论:局部联合应用雷帕霉素滴眼液与环孢霉素A滴眼液能显著延长角膜植片存活时间,抑制大鼠角膜移植排斥反应,具有协同作用。AIM： To evaluate the combined effect of rapamycin ophthalmic solution and cyclosporin A ophthalmic solution on allogeneic transplantation in a rat model. METHODS： SD rats were used as recipients, and Wistar rats were used as donors. The animals were randomly assigned to 4 groups. Group A： control （ophthalmic solution solvent 100μL）; group B： 2g/L rapamycin ophthalmic solution 100μL; group C： 10g/L cydosporin A ophthalmic solution 100μL; group D： 2g/L rapamycin ophthalmic solution 50μL and 10g/L cyclosporin A ophthalmic solution 50μL, 4 times every day. The treatment was started on 2d after operation, and the animals were administered until rejection. The grafts were inspected by slit-lamp microscope and the corneal survival time was recorded. The pathologic changes were measured by light microscope. RESULTS： Graft survival in each treated group was significantly prolonged compared with the control group （P〈 0.01）. However, the combined therapy （group D） was significantly superior compared with group B and C （P〈0.05, P〈 0.01）. The histopathological findings showed that the inflammation cells, neovascularity in each treated group were significantly fewer than that in control group at 14d after operation. CONCLUSION： This is the first study on the efficacy of a double drug regimen with rapamycin ophthalmic solution and cyclosporin A ophthalmic solution for the control of acute corneal allograft rejection. It indicats that the combined therapy produced synergistic effect.

关 键 词：大鼠 角膜移植 免疫排斥 雷帕霉素 环孢霉素A 滴眼液 

分 类 号：R779.65[医药卫生—眼科]