肿瘤坏死因子-α对体外培养的牛眼小梁细胞MMP_3,MMP_9和TIMP-2表达的影响  

Effect of TNF-α on MMP_3,MMP_9 and TIMP-2 expression in cultured bovine trabecular cells

作　　者：吴琼[1] 张德秀[1] 李琛[1] 齐翔云[1] 

机构地区：[1]西安交通大学医学院第一附属医院眼科,中国陕西省西安市710061

出　　处：《国际眼科杂志》2006年第2期328-331,共4页International Eye Science

摘　　要：目的:观察肿瘤坏死因子-α对体外培养的牛眼小梁细胞MMP3,MMP9和TIMP-2表达的影响,探讨原发性开角型青光眼的发病机制。方法:对牛眼小梁细胞进行原代及传代培养,应用免疫组化方法(NSE,Ⅷ因子相关抗原染色)鉴定细胞,化学及电子透射电镜对细胞进行形态学及生长特性的观察;对传3代的小梁细胞分别施加含TNF-α终浓度为0,12.5,25,50μg/L的培养液,48h后行MMP3和MMP9免疫组化SP染色,结果进行计算机图像分析并进行统计学检验。分组提取细胞培养液用ELASA检测TIMP-2量的变化。结果:体外培养的牛眼小梁细胞表达MMP3及MMP9,TNF-α可增加MMP3及MMP9的表达,并且抑制TIMP-2的表达。结论:TNF-α在一定范围内可以增加MMP3及MMP9的表达(P<0.05),并抑制TIMP-2的表达(P<0.01),故TNF-α可以减少小梁细胞细胞外基质的堆积,使房水引流通畅,对激光小梁成形术的手术原理有一定的说明意义。AIM： To study the effect of tumor necrosis factor-α （TNF-α） on the expression of matrix metalloproteinases （MMPs） in the cultured bovine trabecular cells （BTCs）and approach to pathogenesis and mechanism of primary open angle glaucoma （POAG）. METHODS： BTCs were primarily cultured and subcultured. The third passage cells were incubated with different dosages of TNF-α （0, 12.5, 25 and 50μg/L, final concentration, diluted by DMEM） for 48h. MMP3 and MMP9 were determined by immunohistochemistry method. The results were analyzed by the computer image-analysis system and statistical test. Levels of TIMP-2 in cell media were quantified by ELASA. RESULTS： The cultured BTCs expressed MMP3 and MMP9 . In comparison with the control group, TNF-α increased the expression of MMP3 and MMP9 , but decreased TIMP-2 expression in BTCs. CONCLUSION： In certain extent, TNF-α can decrease the stacking in ECM and the resistance of aqueous outflow in trabecular meshwork.

关 键 词：牛眼小梁细胞 肿瘤坏死因子 基质金属 蛋白酶 组织抑制物 免疫组化 酶联免疫吸附 原发性开角型青光眼 氩激光小梁成形术 

分 类 号：R775[医药卫生—眼科]