噻吩磺隆降解菌FLX的分离鉴定及降解特性  

Separation identification and degradation characteristics of thifensulfuron-methyl degradation bacteria FLX

作　　者：黄星[1] 何健[1] 潘继杰[1] 洪青[1] 李顺鹏[1] 

机构地区：[1]南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室,江苏南京210095

出　　处：《中国环境科学》2006年第2期214-218,共5页China Environmental Science

基　　金：国家自然科学基金资助项目(30500010);江苏省科技攻关项目(BE2003343;BE2002303)

摘　　要：从生产噻吩磺隆的农药厂内土壤中采取土样,经驯化富集后筛选到1株能高效降解噻吩磺隆的细菌FLX,根据表型特征、生理生化特性及16SrDNA分析,鉴定FLX初步为寡养单胞菌(Stenotrophomonas sp.).FLX能在含50mg/L噻吩磺隆的基础盐液体培养基中降解噻吩磺隆,48h降解率达83.34%.FLX降解噻吩磺隆的最适pH值为7.0,最适温度为35℃,在所试的金属离子中,Zn2+、Al3+、Cu2+、Ba2+、Fe3+等对FLX的降解影响较小;Hg2+,Co2+则抑制FLX的生长与降解.酶的定域实验表明,该菌中噻吩磺隆水解酶为胞内酶.A Strain of bacterium FLX capable of highly degrading thifensulfuron-methyl was screened from the soil sample collected from a producing thifensulfuron-rnethyl pesticide plant after taming and enrichment. Based on analysis of phenotype, physiological and biochemical characters and 16S rDNA, FLX was identified preliminarily as Stenotrophomonas sp. FLX could degrade thifensulfuron-methyl in its 50mg/L liquor medium, with 83.34% degrading rate in 48h, and the optimal pH value and temperature of 7.0 and 35℃. In the tested metal ions, Zn^2＋. Al^3＋. Cu^2＋, Ba^2＋, Fe^3＋ had little influence on FLX; while Hg^2＋, Co^2＋ inhibited its growth and degradation. The distribution experiment showed that the thifensulfuron-methyl hydrolysis enzyme in the bacterium was endoenzyme.

关 键 词：噻吩磺隆 寡养单胞菌 生物降解 

分 类 号：X172[环境科学与工程—环境科学]