水稻日本晴与广陆矮4号杂交F_2群体SSR标记偏分离原因探析(英文)  

Analysis of Segregation Distortion of Molecular Markers in F_2 Population of Rice

作　　者：赵兵[1] 邓其明[1] 张启军[1] 李杰勤[1] 叶少平[1] 梁永书[1] 彭勇[1] 李平[1] 

机构地区：[1]四川农业大学水稻所

出　　处：《Acta Genetica Sinica》2006年第5期449-457,共9页

基　　金：This work was supported by the National "863" Project (No.2003AA2120300) and Program for Changjiang Scholars and Innova-tive Research Team in University, PCSIRT(No. IRT0453).

摘　　要：以全基因组测序已经完成的材料粳稻日本晴和完成了第4染色体全序列测序的籼稻广陆矮4号的杂交F2作为构图群体,共90个单株,构建了一张含148个微卫星标记的水稻分子遗传图谱。该F2群体显著偏分离非常高,发现有49个分子标记表现偏分离(P<0.05),占总标记数的33.11%,这些偏分离标记中有36个偏向广陆4号,13个偏向杂合体,没有偏向日本晴的偏分离标记。讨论了配子体基因和孢子体基因导致偏分离的原因,通过已经定位的配子体基因和杂种不育基因分布在偏分离集中的区域来进一步说明配子体基因和杂种不育基因确实是导致偏分离形成的原因,而且还通过未定位的标记分析了偏分离的原因。A genetic linkage map comprising 148 SSR markers loci was constructed using an F2 population consisting of 90 lines derived from a sub-specific cross between a japonica variety Nipponbare and an indica variety Guangluai-4. The F2 population showed high significantly distorted segregations. Among these SSR markers, 49 markers（33.11%） showed the genetics distortion （P 〈 0.05）. Of them, 36 markers deviated toward male parent indica GuangLuAi-4 and 13 markers toward heterozygote, but none toward the female parent Nipponbare. It was found that the segregation distortion might be caused by gametophyte and zygote. Since most gametophyte loci and sterility loci were mapped in segregation distortion regions, it indicated that the segregation distortion may be caused by these gametophyte loci and sterility loci. Finally, this research also analyzed the skewed segregation of some markers, which had not been mapped on chromosome.

关 键 词：水稻 F2群体 SSR标记 偏分离 

分 类 号：S511[农业科学—作物学]