黄连素对高脂饮食肝损伤大鼠肝中NO合成酶及凋亡相关基因的干预与调控  

Modulation of Berberine on the Expression of Nitric Oxide Synthase and Bax, Bcl-2 in Liver Injury Mice Treated by High Fat-High Cholesterol Diet

作　　者：王黎[1] 马瑶[1] 付婷婷[1] 刘颖[1] 王莹[1] 周娟[1] 高天芸[1] 洪蓓蓓[1] 孙玉芹[1] 丁虹[1] 

机构地区：[1]武汉大学药学院,武汉430072

出　　处：《中国药师》2006年第5期387-389,共3页China Pharmacist

基　　金：国家973重大基础研究专项(2002ccc00300)

摘　　要：目的:研究高脂饮食肝损伤中结构型(cNOS)、诱导型(iNOS)一氧化氮(NO)合成酶及凋亡相关基因Bax、Bcl-2表达的变化,在进一步探讨脂肪性肝损伤机制的同时观察黄连素的干预调控作用。方法:建立高脂饮食大鼠肝损伤模型,ig给予黄连素20,40,60 mg·kg-1,测定血中TC,TG,HDL,LDL含量及ALT,GST活性和肝中NO含量;免疫组化方法观察cNOS,iN- OS,Bax,Bcl-2的表达。结果:黄连素可使血中TC,TG,LDL含量及sALT,sGST活性下降,呈良好的剂量依赖关系,对HDL无明显作用;能显著抑制肝组织中NO的生成;黄连素对高脂饮食大鼠肝组织中iNOS,Bax表达有一定的抑制作用,但作用无显著性差异;可显著上调肝组织中cNOS及Bcl-2的表达,但不呈剂量依赖关系。结论:黄连素可保护南脂饮食所致的肝损伤,可显著上调抗凋亡基因Bcl-2的表达,提示黄连素可能通过抗肝细胞凋亡保护高脂饮食引起的肝细胞损伤。Objective： To investigate the modulation of Berberine on the expression of nitric oxide synthase and Bax, Bcl-2 in liver injury mice treated by high fat-high cholesterol diet. Method： Liver injury model was induced by high-fat-high-cholesterol. Berberine was administrated to mice with 20, 40, 60 mg·kg^-1 , respectively, ig, once a day. Mice were sacrificed at the end of the 6th week, the effects of Berberine on the expression of inducible, constitutive nitric oxide synthase and Bax, Bcl-2 gene were assessed by immunohis to chemistry , and the nitric oxide （NO） production, alanine transaminase（sALT） activity,glutathione S-transferase（sGST） activity in serum were determined. Result： Compared with the normal mice, the NO production,TC ,TG,LDL content and sALT, sGST level were increased significantly, no change of HDL was found; iNOS, Bax expression were up-regulation, cNOS expression and Bcl-2 expression were down-regulation in liver injury mice. The Berberine could obviously reduce NO production and sALT, sGST level, increase the expression of cNOS and Bcl-2, and no effect on iNOS, Bax expression was found. Conclusion： Berberine can alleviate the liver injury by modulation of the expression of cNOS, Bcl-2 to prevent apoptosis.

关 键 词：黄连素 一氧化氮合成酶 凋亡 高脂饮食 肝损伤 

分 类 号：R285.5[医药卫生—中药学]