基因芯片技术检测脑出血大鼠血肿周围组织早期基因表达(英文)  

Detection on early gene expression of tissues around hematom in rats with cerebral hemorrhage with the technique of DNA microarray

作　　者：吴碧华[1] 胡常林[2] 

机构地区：[1]川北医学院附属医院神经内科,四川省南充市637007 [2]重庆医科大学第二附属医院神经内科,重庆市400010

出　　处：《中国临床康复》2006年第20期173-175,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:任何生理状况的改变和表型的变化,最初的基础激发点是基因表达的改变,基因芯片技术是利用碱基配对的原理来快速、大量筛选目标基因的新方法,能整体宏观地研究生物体基因的表达及功能。目的:应用基因芯片技术研究大鼠脑出血早期差异表达基因,为探讨脑出血的病理机制奠定理论基础。设计:随机对照实验。单位:川北医学院附属医院神经内科。材料:实验于2002-10/2003-12在川北医学院附属医院完成。选取20只无特殊病原体级Wistar大鼠,雌雄各半,体质量220～260g,由重庆医科大学实验动物中心提供,将全部大鼠随机分成对照组和脑出血组,每组10只。方法:采用Ⅶ型胶原酶立体定位法制备大鼠急性脑出血动物模型,模型建立后4h取血肿周围组织和相同部位的正常脑组织进行基因芯片对照检测,用扫描仪扫描芯片荧光信号并进行计算机分析,用反转录-聚合酶链反应来考证基因表达谱的结果。主要观察指标:大鼠脑组织基因芯片检测结果及基因反转录-聚合酶链反应的计算结果。结果:大鼠急性脑出血后4h有差异表达基因129个,上调基因114个,下调基因15个,这些基因主要涉及到应激和免疫应答、细胞凋亡、能量代谢及信号转导。有关炎性损害的基因上调最为明显。反转录-聚合酶链反应计算结果显示,基因表示水平与芯片检测结果相符,说明基因芯片所建立的基因表达谱具有相当的可靠性。结论:脑出血早期存在多个差异表达基因,这些差异表达的基因可能在出血性脑损伤中发挥重要作用。BACKGROUND： Changes of physiological structure, changes of phenotype and first basic excision are all the changes of gene expression. The technique of DNA mieroarray is a new method to filtrate target genes fleetly and largely by using the theory of base-partnershin, which can holistieally and magnificently study the expression and function of organics genes. OBJECTIVE： To study early differential expression genes of rats with cerebral hemorrhage with DNA microarray and establish academic foundation for exploring mechanism of cerebral hemorrhage. DESIGN： Randomized controlled research. SETTING： Department of Neurology, Affiliated Hospital of North Siehuan Medical College. MATERIALS： The experiment was conducted at the Affiliated Hospital of North Siehuan Medical College from October 2002 to December 2003. Twenty Wistar rats, of either gender, with body mass of 220-260 g, without special pathogen, provided by Experimental Animal Center of Chongqing University of Medical Sciences, were selected and randomly divided into control group and cerebral hemorrhage group, with 10 in each group. METHODS： Animal models with cute cerebral hemorrhage of rats were established with type Ⅶ eollagenase tridimensional localization method, and 4 hours later tissues around hematom and normal cerebral tissue at the same part were detected with gene chip. Fluorescent signal was scanned with scanning apparatus and analyzed with computer. Result of genie expressive pattern was researched with reverse transeriptase-polymerase chain reaction （RT-PCR）. MAIN OUTCOME MEASURES： Result of gene chip in cerebral tissue of rats and result of RT-PCR. RESULTS： Four hours after acute cerebral hemorrhage, 129 differential expression genes were screened out, in which there were 114 up-regnlation genes and 15 down-regnlation genes. Those genes were mostly related to the following aspects： stress, immunological response, apoptosis, energy metabolism and signal transmitting. Genes related with inflammatory impairment

关 键 词：脑血管意外 大鼠 基因表达谱 炎症 

分 类 号：R743[医药卫生—神经病学与精神病学]