周围神经端侧吻合新方法大鼠实验模型的建立  

Establishment of a new animal model for end-to-side anastomosis of peripheral nerves

作　　者：朱小雷[1] 张云峰[2] 孙占胜[1] 陈振强[3] 

机构地区：[1]山东大学医学院山东省立医院手足外科,济南250021 [2]青岛市立医院骨科 [3]山东大学医学院

出　　处：《中华创伤杂志》2006年第5期372-376,共5页Chinese Journal of Trauma

摘　　要：目的设计制作端侧吻合新方法的动物实验模型,并进行检测。方法实验用W istar大白鼠,共28只。按拟行神经吻合的方式,将大鼠的左下肢定为实验侧,右下肢定为对照侧。实验侧:切断大鼠左侧胫神经,将胫神经的近断端与腓总神经端侧吻合;对照侧:切断大鼠右侧腓总神经,将腓总神经的远断端与胫神经端侧吻合。3个月后,进行电生理检查,比较两侧固定刺激量的波幅、潜伏期、最大波幅。比较实验侧吻合口远、近端的腓总神经纤维计数。结果实验侧与对照侧相比,波幅、潜伏期、最大波幅差异无统计学意义。实验侧吻合口远侧的神经纤维计数多于近侧神经纤维计数(P<0.05)。结论端侧吻合新方法的轴突再生机制为胫神经轴突发芽,再生的轴突通过吻合口沿着腓总神经外膜或束膜管道向靶肌肉生长。Objective To design a new animal modal for end-to-side anastomosis of peripheral nerves and analyze the efficiency. Methods The experiment involved 28 Wistar rats, of which the left legs were set as experimental group and the right ones as control group based on mode of nerve anastomosis. In experimental group, the tibial nerves were cut off for anastomosis of the proximal end of the tibial nerves with the distolateral side of the common peroneal nerve. While in control group, the common pero- neal nerves were cut off to anostmose the distal end of the peroneal nerve with the distolateral side of the tibial nerves. Electrophysiological examination was done three months later to observe and compare wave amplitude, latent period and maximal wave amplitude at set stimulus volume in both groups and compare the fiber number at distal and proximal ends of the common peroneal nerves. Results There was no significant statistical difference in regard of amplitude, latent period and maximal wave amplitude in both groups. However, the fiber number at distal end of the stoma was much more than that at proximal end in the experimental group, with statistical difference （P 〈 0.05）. Conclusion The mechanism of end-toside anastomosis of peripheral nerves may be that the tibial nerves pullulate into the target muscle along epineurium or fascicular membrane of the common peroneal nerves through stoma.

关 键 词：周围神经 吻合术 外科 动物模型 轴突 神经再生 

分 类 号：R-332[医药卫生]