食管癌survivin、caspase-3表达与细胞凋亡的关系  

Relationship between expression of survivin mRNA, caspase-3 and cell apoptosis in esophageal carcinoma

作　　者：王克文[1] 张桂英[2] 

机构地区：[1]郴州市第一人民医院,湖南郴州423000 [2]中南大学湘雅医院,湖南长沙410000

出　　处：《现代肿瘤医学》2006年第5期560-563,共4页Journal of Modern Oncology

摘　　要：目的探讨survivinmRNA、caspase-3蛋白在食管鳞癌中的表达及其与细胞凋亡的关系。方法分别采用RT-PCR和免疫组化技术检测38例食管鳞癌及其对应的正常组织中survivinmRNA和caspase-3蛋白的表达,运用TUNEL方法检测细胞凋亡。结果73.68%食管鳞癌组织呈survivinmRNA阳性表达,显著高于正常组织,并与食管鳞癌的分化程度、临床分期有关,与年龄、性别、淋巴结转移无关;caspase-3蛋白在食管鳞癌和正常组织中的阳性表达率分别为26.32%和89.47%,差异有显著性,并与食管鳞癌的分化程度有关,与年龄、性别、淋巴结转移和临床分期无关;在食管鳞癌组织中,survivinmRNA和Caspase-3蛋白表达呈负相关;survivinmRNA阳性的食管鳞癌组中细胞凋亡指数明显低于survivinmRNA阴性组,(P<0.01),caspase-3蛋白阳性的食管鳞癌组中平均细胞凋亡指数明显高于caspase-3蛋白阴性组(P<0.01)。结论survivin、caspase-3参与了食管鳞癌的发生、发展,可以作为食管鳞癌预后的指标,survivin通过抑制caspase-3的活性而发挥其抑制细胞凋亡的作用是其主要的分子机制。Objective： To investigate the relationship between expression of survivin mRNA and caspase - 3 and cell apoptosis in esophageal cancer. Methods： We detected expression of survivin mRNA in esophageal squamous cell carcinoma tissues and the corresponding esophageal normal tissues by reverse transeriptase polymerase chain reaction. Immunohistochemistry was used to detect the expression of caspuse - 3 protein in all samples. Carcinoma cell apopto- sis was assessed using terminal deoxynucleotide transferase- mediated dUTP nick end labeling （TUNEL）. Results： The results showed that survivin mRNA expression was detected in （73.68%） esophageal squamous cell cancer tissues and was significantly higher than in normal tissues, with significant difference in cell differentiation and clinical stages, without significant difference in age, gender and lymph node metastasis. The caspase - 3 protein was detected in 10 of 38 （26.32%） esophageal cancer tissues and in 34 of 38 （89.47%） esophageal normal tissues, with signiilcant difference. Expression of caspase - 3 was significantly associated with histological stages, but not with age, gender, lymph node -metastasis, and clinical stages. Survivin expression in esophageal carcinoma tissues was inversely related to caspase - 3 expression. Average apoptosis index of survivin mRNA positive esophageal squamous cell carcinoma tissues group was significantly lower than that of survivin mRNA negetive group. Average apoptosis index of caspase- 3 protein positive esophageal squamons cell carcinoma tissues group was significantly higher than that of caspase -3 protein negetive group. Condusion：Survivin and caspase -3 may play a significant role in the carcinogenesis of the esophageal squamous cell carcinoma and maybe associated with the prognosis of the cancer. Survivin exert probably the use of restraining cell apoptosis by the pathway of caspase - 3.

关 键 词：食管癌 SURVIVIN CASPASE-3 细胞凋亡 分子机制 

分 类 号：R735.1[医药卫生—肿瘤]