专性嗜碱芽孢杆菌Bacillus pseudo firmus的基因导入方法  

Transformation methods of alkalophilic Bacillus pseudofirmus

作　　者：刘敏胜[1] 邢新会[1] 

机构地区：[1]清华大学化学工程系生物化工研究所,北京100084

出　　处：《化工学报》2006年第4期922-926,共5页CIESC Journal

基　　金：国家自然科学基金项目(20176025);国家教育部博士点基金项目(20020003057).~~

摘　　要：Aiming at development of transformation methods for alkalophilic Bacillus pseudofirmus JCM 9141 (growth at pH>10), screening conditions of transformants by using antibiotics were first examined to overcome the antibiotic inactivation at high pH. Then, different transformation methods including competent cells, protoplast and electroporation were studied, respectively. The transformation efficiencies of competent cells and protoplast were low, while a modified electroporation method achieved higher transformation efficiency under 3000 V·cm -1. This method was also applicable to other alkalophilic Bacillus sps.Aiming at development of transformation methods for alkalophilic Bacillus pseudofirmus JCM 9141 （growth at ph〉10）, screening conditions of transformants by using antibiotics were first examined to overcome the antibiotic inactivation at high pH. Then, different transformation methods including competent cells, protoplast and electroporation were studied, respectively. The transformation efficiencies of competent cells and protoplast were low, while a modified electroporation method achieved higher transformation efficiency under 3000 V · cm^-1. This method was also applicable to other alkalophilic Bacillus sps.

关 键 词：嗜碱微生物 芽孢杆菌 转化 感受态细胞 原生质体 电穿孔 

分 类 号：TQ920[轻工技术与工程—发酵工程]