姬松茸菌丝体多糖的分离纯化及抗疲劳研究  

STUDY OF ISOLATION AND PURIFICATION OF POLYSACCHARIDES FROM FRESH MYCELIA OF AGARICUS BLAZEI MUSSILL AND ITS ANTI-FATIGUE EFFECT

作　　者：刘新海[1] 冯培勇[1] 史亚丽[2] 杨立红[1] 蔡德华[1] 

机构地区：[1]烟台师范学院生命科学院,山东烟台264025 [2]烟台师范学院体育学院,山东烟台264025

出　　处：《西南农业大学学报（自然科学版）》2006年第2期190-194,共5页Journal of Southwest Agricultural University

基　　金：山东省科技厅资助项目[鲁农科字(2001)500号]

摘　　要：通过液体深层发酵获得姬松茸(Agaricus blazeiMurrill)菌丝体。用水提法提取姬松茸菌丝体多糖(即胞内多糖),并通过葡聚糖凝胶SephadexG-200分离纯化出2种姬松茸多糖组分ABP-I和ABP-II,紫外光谱检测表明2种多糖均不含核酸和蛋白质。红外光谱和高效液相色谱测定结果表明,ABP-I和ABP-II均为含有葡萄糖醛酸的D-葡聚糖。对力竭小鼠抗疲劳作用研究结果表明,姬松茸胞内多糖具有明显的抗疲劳作用。Two polysaccharides （ ABP- Ⅰ and ABP- Ⅱ ） were isolated and purified from the fresh mycelia of Agaricus blazei Mussill. UV spectroscopic examination revealed that the polysaccharides were nucleic acid-free and protein-free. Infra-red and HPLC experiments showed that ABP Ⅰ and ABPⅡ are D-polyglucosans containing glucuronic acid. A test with mice demonstrated that ABPⅠ and ABP Ⅱ have an anti-fatigue function.

关 键 词：姬松茸 胞内多糖 抗疲劳 

分 类 号：S646.199[农业科学—蔬菜学]