强力霉素对胃癌细胞SGC-7901基质金属蛋白酶-2和基质金属蛋白酶组织抑制因子-2表达的影响  

Effects of Doxycycline on Expression of Matrix Metalloproteinase-2 and Tissue Inhibitor of Metalloproteinase-2 in Gastric Cancer Cells SGC-7901

作　　者：谢兆兰[1] 朱尤庆[1] 林小玲[1] 汤小燕[1] 

机构地区：[1]武汉大学中南医院消化内科,430071

出　　处：《胃肠病学》2006年第4期202-206,共5页Chinese Journal of Gastroenterology

摘　　要：背景:强力霉素对结肠癌细胞的分化和抑制作用已有报道,但其对胃癌细胞的作用尚未见报道。目的:观察强力霉素对人胃癌细胞SGC-7901的生长抑制作用及其对基质金属蛋白酶(MMP)-2和基质金属蛋白酶组织抑制因子(TIMP)-2表达的影响,探索胃癌治疗的新方法。方法:采用不同浓度的强力霉素作用于胃癌细胞SGC-7901,以噻唑蓝(MTT)法测定其细胞毒作用;逆转录聚合酶链反应(RT-PCR)法半定量测定MMP-2和TIMP-2mRNA的表达;免疫组化法观察MMP-2蛋白的表达。结果:强力霉素可抑制胃癌细胞SGC-7901的生长,具有浓度和时间依赖性(P<0.01)。强力霉素可下调MMP-2mRNA和MMP-2蛋白的表达,上调TIMP-2mRNA的表达,具有浓度依赖性(P<0.05)。结论:强力霉素能抑制胃癌细胞SGC-7901的生长,其作用机制可能与下调MMP-2表达、上调TIMP-2表达有关。Background： Doxycycline could inhibit the proliferation of colonic cancer cells. However, its effect on gastric cancer cells has not been reported. Aims： To observe the inhibitory effect of doxycycline on the growth of human gastric cancer cells （SGC-7901） and the effect on expression of matrix metalloproteinase （MMP）-2 and tissue inhibitor of metalloproteinase （TIMP）-2 and to explore new therapeutic approach in the treatment of gastric cancer. Methods： SGC- 7901 cells were treated with different concentrations of doxycycline and its growth inhibitory effect was detected by methyl thiazolyl tetrazolium （MTT） method. The mRNA expression of MMP-2 and TIMP-2 were analyzed by semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR） method; the expression of MMP-2 protein was examined by immunohistochemistry. Results： The growth of SGC-7901 cells was inhibited by doxycycline in a time- and dosedependent manner （P〈0.01）. Doxycycline decreased the expression of MMP-2 mRNA and MMP-2 protein, but increased the expression of TIMP-2 mRNA dose-dependently （P〈O.05）. Conclusions： Doxycycline can inhibit the proliferation of SGC-7901, which may be related to its effect of down-regulating MMP-2 and up-regulating TIMP-2.

关 键 词：强力霉素 胃肿瘤 细胞系 基质金属蛋白酶-2 基质金属蛋白酶组织抑制因子-2 逆转录聚合酶链反应 

分 类 号：R735.2[医药卫生—肿瘤]