应激大鼠幽门区一氧化氮和神经元型一氧化氮合酶与胃内胆汁反流的关系  

Correlations between Nitric Oxide, Neuron Nitric Oxide Synthase and Gastric Bile Reflux in Stressed Rats' Pylorus

作　　者：刘兴栋[1] 罗和生[1] 刘艳[1] 李玲[1] 

机构地区：[1]武汉大学人民医院消化内科,430060

出　　处：《胃肠病学》2006年第4期207-210,共4页Chinese Journal of Gastroenterology

摘　　要：背景:幽门区一氧化氮(NO)与幽门括约肌舒张功能密切相关,但从形态学上探讨神经元型一氧化氮合酶(nNOS)与胃内胆汁反流关系的研究却较少。目的:探讨应激大鼠幽门区NO和nNOS含量变化对胃内胆汁反流的影响。方法:55只大鼠随机分为正常对照组(n=10)、实验组(n=30)和免疫组化组(n=15),后两组以水浸束缚应激诱导应激性溃疡模型。实验组于应激开始后2、4、5、6、8和10h各取5只大鼠,分别检测胃内胆汁酸浓度和胃黏膜溃疡指数(UI,Guth评分),以生化试剂盒检测幽门区NO含量。免疫组化组于应激开始后0、4和6h各取5只大鼠,以免疫组化SP法结合图像分析检测幽门区nNOS免疫反应阳性产物的平均光密度值。结果:应激性溃疡大鼠幽门区NO含量于应激结束后1h达到峰值(P<0.01);应激结束后2h,胃内胆汁酸浓度达到峰值(P<0.01);胃黏膜UI于应激结束后4h达到峰值(P<0.01)。免疫组化染色显示应激性溃疡大鼠胃内胆汁反流增加时,幽门区肌间神经丛nNOS免疫反应阳性产物平均光密度值显著增高(P<0.01)。结论:NO能促进应激大鼠胃内胆汁反流,NO和nNOS参与了幽门括约肌舒张功能的调控。Background： The pyloric area nitric oxide （NO） is closely correlated with the relaxation function of pyloric sphincter, but few morphological studies have been carried out on the relationship between neuron nitric oxide synthase （nNOS） and bile reflux in the stomach. Aims： To appraise the effects of pyloric NO and nNOS on bile reflux in stressed rats＇ stomach. Methods： Fifty-five rats were randomly divided into three groups： normal control group （n=10）, experimental group （n=30） and immunohistochemical staining group （n=15）. Stress ulcer model was constructed by water-immersion restraint in the latter two groups. In the experimental group, the gastric bile acid, ulcer index （UI） and pyloric NO were measured at 6 time points： 2, 4, 5, 6, 8 and 10 hours after the beginning of stress in five rats of each, respectively. In immunohistochemical staining group, the mean optical density of nNOS-positive products at the pyloric sphincter was studied by SP immunohistochemistry and image analysis 0, 4 and 6 hours after starting the stress in five rats of each. Results： The pyloric NO reached the peak 1 hour after stress （P〈0.01）, and the gastric bile acid and UI reached the maximum 2 and 4 hours after stress, respectively （P〈0.01）. Accompanied by the increased bile reflux, the mean optical density of nNOS-positive products significantly increased in the nerve plexus of pyloric sphincter （P〈0.01）. Conclusions： NO can promote bile reflux in the stressed rat＇s stomach. NO and nNOS affect and regulate the relaxation function of pyloric sphincter.

关 键 词：一氧化氮 神经元型一氧化氮合酶 胆汁反流 应激 

分 类 号：R57[医药卫生—消化系统]