如何建立成年大鼠心肌细胞钙超载模型  

How To Build Calcium Overload Model of Adult Rat Ventricular Cardiomyocytes

作　　者：陈焕文[1] 石应康[1] 李勇刚[1] 刘小菁[2] 王树人[3] 

机构地区：[1]四川大学华西医院胸心外科,四川成都610041 [2]四川大学华西医院心血管疾病研究室,四川成都610041 [3]四川大学基础医学院病理生理教研室,四川成都610041

出　　处：《华西医学》2006年第2期308-309,共2页West China Medical Journal

摘　　要：目的：探讨建立成年大鼠心肌细胞钙超载模型的方法。方法：Langendorff灌流，分离钙耐受成年大鼠心肌细胞，采用不同浓度钙离子导入剂-伊屋诺霉素（ionomycin）作用心肌细胞1小时，用荧光分光光度计测定心肌细胞内的游离钙浓度。结果：1．分离的活性心肌细胞比率约70～80％，杆状、横纹清晰。2．实验组1（ionomycin0．5μmol／L）心肌细胞[Ca^2＋]1明显高于对照组（282．07±25．19 vs 115±21．21nmol／L，P〈0．05）；实验组2（ionomycin 1．0μmol／L）心肌细胞[Ca^2＋]1（376．35±29．44）显著高于正常对照组（P〈0．05）。结论：通过本方法可以建立成年大鼠心肌细胞钙超载模型。Objective： To develop the calcium overload model with adult rat ventricular cardiomyoeytes.Methods： Calcium tolerant cardiomyocytes were isolated by Langendorff perfusion. Then, cardiomyocytes were exposed to ionomycin for 1 hour, and cytosolic calcium was measured with spectrofluorometer. Results： Viability of freshly isolated adult rat ventricular cardiomyocytes, which were rod- shaped with clear cross- striations, was 60% - 80% . Cytosolic calcium of cardiomyocytes stimulated by 0.5μmol/L or 1.0μmol/L ionomycin increased significantly by 145 % （ P 〈 0.05, vs control） and 226 % （ P 〈 0.05, vs control） respectively, Conclusion： Calcium overload model of adult rat ventricular cardiomyocytes can be built by ionomycin.

关 键 词：大鼠 心肌细胞 钙超载模型 

分 类 号：R-332[医药卫生]