静脉桥狭窄实验动物模型的建立  

Constitution of vein graft stenosis model

作　　者：潘文标[1] 郑家豪[1] 连锋[1] 吴学军[1] 张谷兰[1] 王小妹[1] 

机构地区：[1]上海第二医科大学附属仁济医院胸心外科,上海200001

出　　处：《现代医学》2006年第1期6-8,共3页Modern Medical Journal

基　　金：上海市教委资助项目(03BK21);上海市浦东新区科技局资助项目(Pkj2002-60)

摘　　要：目的建立一种与人冠状动脉旁路移植手术（coronary artery bypass graft，CABO）后大隐静脉桥狭窄病理过程相似的动物模型。方法20～25k普通长白猪8只．后腿外侧纵切口，“no-touch”技术剥取大隐静脉3cm，与颈总动脉进行端端吻合。术后口服肠溶阿司匹林150mg·d^-1抗凝。术后28d取下静脉桥，采用计算机图像处理系统分析新内膜厚度、新内膜面积，（新内膜＋中膜）面积（I／M）。结果肉眼观察，8只猪大隐静脉桥管壁均增厚，管腔狭窄；光镜下见静脉桥新内膜形成，新内膜厚度为（0．5009±0．1355）mm，I／M为（0．4651±0．0512）。结论猪大隐静脉-颈总动脉静脉桥模型可成功模拟人CABG术后大隐静脉桥的病理变化。Objective To constitute a vein graft model that can mimic the pathology of human saphenous vein graft. Methods Studies were performed by using ＂white race＂ pigs （ n = 8）, weight 20 - 25 kg. Animals were subjected to premedication, anesthesia. A longitudinal incision was made on the outer aspect of the hind leg to ex ＂no pose and remove approximately 3 cm in length of the long saphenous vein with a touch＂ technique. A 3 cm segment of one common carotid artery was isolated through a longitudinal neck incision, and both cut ends were then beveled to about 45 degree. One end of a segment of reversed saphenous vein was similarly beveled and then anastomosed to the carotid artery by an end-to-end anastomotic technique under optical magnification with continuous 8-0 polyprolene suture. The second anastomosis was performed similarly. Proximal anastomosis was always done first.The graft was de-aired through the suture line before the suture of the second anastomosis was tied. The animals were fed a normal chow diet and aspirin, 150 mg·d^-1 for 28 days. Grafts were then removed, fixed with the use of 10% buffered formal saline solution, and then processed for wax embedding. Transverse （5μm） sections were stained with haematoxylin-eosine, and examined under a light microscope. Vessel wall dimensions were measured by computer-aided planimentry. Results All the grafts were patent, in that all saphenous vein graft wall thickening and lumen stenosis were examined by baked-eye. Neointimal proliferation was examined under a light microscope. Neointimal thickening was equivalent to （0. 500 9 ± 0.135 5） mm, neointimal area divided by neointimal area and medial area was equivalent to 0.465 1 ± 0.051 2. Conclusion We constituted a porcine saphenous vein into tarotid artery interposition grafting medel which can mimic the pathology of human saphenous vein graft.

关 键 词：静脉桥狭窄 动物模型 猪 实验 

分 类 号：R-332[医药卫生]