产1,3-丙二醇基因工程菌发酵条件的研究  

Study of the fermentation conditions for the recombinant microorganism producing 1,3-propanediol

作　　者：韦旭钦[1] 陈发忠 罗兆飞 韦宇拓[1] 周文广[1] 黄日波[1] 

机构地区：[1]广西大学生命科学与技术学院发酵与酶工程研究所,南宁530005 [2]广西南宁中诺生物工程有限责任公司,南宁530003

出　　处：《生物加工过程》2006年第1期39-43,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家"863"高科技资助项目(2003AA001039)

摘　　要：利用途径工程的方法,将来源于克雷伯氏菌(Klebsiella pneumoniae)的甘油脱水酶基因dhaB和1,3-丙二醇氧化还原酶基因dhaT构建成多顺反子重组质粒pSE-dhaB-dhaT并在大肠杆菌JM 109中进行表达,在大肠杆菌中构建一条新的产1,3-丙二醇代谢途径。研究表明,重组菌株JM 109/pSE-dhaB-dhaT在微好氧条件下,尝试用廉价的乳糖为诱导物、维生素B12为辅酶,可以将甘油转化为1,3-丙二醇,产量达15.34 g/L,甘油转化率为35.7%,对低成本生产1,3-丙二醇作了有益的探索。Based on the principle of the pathway engineering, a novel pathway of producing 1,3-propanediol was built in E. coll. The dhaB gene encoding glycerol dehydratase （DHAB） and the dhaT gene encoding 1, 3-propanediol oxidoreductase （DHAT） were cloned from Klebsiella pneumoniae and then inserted into expression vector pSE380 to obtain recombinant polycistron plasmid pSE-dhaB-dhaT. This polycistron was transformed into E.coli JM109. The result showed that the recombinant microorganism JM109/pSE-dhaB-dhaT could convert glycerol to 1,3-propanediol directly under the induced conditions of lactose and vitamin B12 in the fermentor,and the maximal concentration of 1,3-propanediol was 15.34 g/L at 60 h, conversion rate of glycerol was 35.7 % under the microaerobic condition.

关 键 词：1 3-丙二醇 重组菌 发酵条件 

分 类 号：Q78[生物学—分子生物学]