高固液比体系中酶催化合成Z-Phe-Leu-OMe的研究  

Enzymatic Synthesis of Z-Phe-Leu-OMe in A High Solid-Liquid Ratio System

作　　者：黄振龙[1] 宗璟[1] 陈英文[1] 沈树宝[1] 

机构地区：[1]南京工业大学制药与生命科学学院,南京210009

出　　处：《食品与生物技术学报》2006年第2期20-23,共4页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(20376034);江苏省自然科学基金项目(BK2003084)

摘　　要：以模型二肽Z-Phe-Leu-OMe的合成为对象讨论了高固液比条件下酶催化体系的性质。研究了固液比高低对二肽产率的影响,测定了高固液比条件下随着反应进行体系pH的变化,尝试提出该类体系pH变化的机理,并采用KHCO3和CaCO3调节该体系的pH来提高催化效率。Enzymatic synthesis of model dipeptide Z-Phe-Leu-OMe in a high solid-liquid ratio system was investigated. Effects of solid-liquid ratio on the dipeptide yield were analyzed. In addition, the pH change of the system during the reaction was monitored, and the mechanism of this change was presented. The catalytic efficiency of Z-Phe-Leu-OMe enzymatic synthesis was further promoted by adjusting pH of the system through addition of KHCO3 and CaCO3.

关 键 词：高固液比 模型二肽 酶催化 pH值 

分 类 号：Q814.9[生物学—生物工程]