超高压提取山楂叶中黄酮类化合物  

High hydrostatic pressure extraction of flavonoids from Hawthorn leaves

作　　者：李宏伟[1] 张守勤[1] 窦建鹏[1] 奚玉石[1] 

机构地区：[1]吉林大学生物与农业工程学院

出　　处：《吉林大学学报（工学版）》2006年第3期438-442,共5页Journal of Jilin University:Engineering and Technology Edition

基　　金：国家自然科学基金资助项目(30472135)

摘　　要：为了研究超高压提取山楂叶中黄酮类化合物的最佳工艺,采用超高压技术常温提取,正交试验优化,分光光度法检测山楂叶中黄酮类化合物的含量。结果表明:超高压提取山楂叶中黄酮类化合物的最佳提取工艺参数为:提取溶剂为50%乙醇,提取压力为400 MPa,料液比为1∶45,提取温度为60℃,提取时间为3 m in。该超高压提取工艺具有提取效率高、时间短、杂质含量少等优点。High hydrostatic pressure extraction （HHPE） technique was used to extract flavonoids from Hawthorn leaves at normal temperature. The content of flavonoids was determined by UV-spectrophotometry. Parameters that may influence the extraction process were investigated. Orthogonal test was applied to obtain the optimum extraction condition, which was as follows ： solid dissolved in 50% ethanol, ratio of raw Hawthorn leaves （g） to solvent （ml） of 1：45, temperature of 60℃, extraction pressure of 400 MPa and extraction time of 3 min. Results of this work show that HHPE possesses several advantages over other extraction methods, such as higher efficiency, shorter extraction time, and less impurity in the extracted flavonoids.

关 键 词：生物药物学 山楂叶 黄酮类化合物 超高压提取 正交实验 

分 类 号：R284.2[医药卫生—中药学] TQ914.1[医药卫生—中医学]