食管癌组织芯片p53、p16和环氧合酶-2表达的研究  

Expression of p53, p16 and cyclooxygenase-2 in esophageal cancer detected by microarray

作　　者：李岚[1] 虞朝辉[1] 刘有恃[1] 章宏[1] 张宝峰[2,3] 方静[2] 周琼[2] 胡莺[2] 郜恒骏[2] 厉有名[1] 

机构地区：[1]浙江大学医学院附属第一医院消化科,杭州310003 [2]生物芯片上海国家工程研究中心 [3]上海芯超生物科技有限公司

出　　处：《中华消化杂志》2006年第3期163-166,共4页Chinese Journal of Digestion

基　　金：国家863计划功能基因组与生物芯片重大专项(2002AA2Z2021)

摘　　要：目的食管癌的发生发展是多步骤、多基因变化的演化过程,本研究利用高通量的组织芯片技术,对食管癌组织及癌旁组织的p53、p16和环氧合酶(COX)-2蛋白异常表达进行分析,探讨其相关性及临床意义。方法利用组织芯片技术结合免疫组化法检测86例食管癌组织、40例癌旁组织中 p53、p16、COX-2蛋白的表达。结果食管癌组织中p53、COX-2的阳性表达率均显著高于癌旁组织(P< 0．05)。食管癌组织中p16阳性表达率为5．81％,癌旁组织中没有发现p16蛋白表达,差异无统计学意义。p53与p16、p53与COX-2、p16与COX-2蛋白表达均存在差异(P<0．05)。p53或COX-2表达阳性时组织芯片病理类型为癌性的概率增加,但p16、p53和COX-2三者不存在交互作用。结论 p53、 COX-2对预测和早期诊断食管癌具有重要意义。Objectives Esophageal cancer development and progression is driven by the accumulation of genetic changes. In this study, we used tissue microarray to survey the expression of p53, p16 and COX-2 in esophageal cancer progression and their clinical implication. Methods We tested p53, p16 and COX-2 proteins by immunohistochemistry and tissue microarray in 86 specimens from different stages of esophageal cancer and 40 specimens from adjacent non-cancer tissue. Results The expression of p53 and COX-2 was significantly higher in tumor tissue than that in non-tumor ones. As for the expression of p16, no significant difference was found between tumor and adjacent tissue. An obvious relation was observed among p53, p16 and COX-2 expression that esophageal carcinogenesis was highly correlated with the positive expression of p53 or COX-2, however, no reciprocal relationship to neoplastic progression was recognized with p53, p16 and COX-2. Conclusion We observed that the tissue with the positive expression of p53 or COX-2 was more likely to develop esophageal cancer. Further work will verify the hypothesis that the expression level of p53 and COX-2 in biopsy specimen is applicable to predict early outset of esophageal cancer.

关 键 词：食管癌 组织芯片 P53 P16 环氧合酶-2 

分 类 号：R735.1[医药卫生—肿瘤]