实验性脑出血后水通道蛋白4的表达变化(英文)  

Changes of aquaporin-4 expressions after experimental intracerebral hemorrhage

作　　者：李燕华[1] 孙善全[2] 李吕力[1] 王铁建[1] 

机构地区：[1]广西壮族自治区人民医院神经内科,广西壮族自治区南宁市530021 [2]重庆医科大学神经生物学研究室,重庆市400016

出　　处：《中国临床康复》2006年第18期178-181,F0003,共5页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助(30070247)~~

摘　　要：背景:水通道蛋白4可能是导致脑水肿形成的重要调节因素之一,但水通道蛋白4是否参与脑出血后脑水肿的形成尚未见报道。目的:观察出血性脑水肿病理过程中水通道蛋白4的表达情况,探讨其与脑水肿形成的关系。设计:随机对照动物实验。单位:广西壮族自治区人民医院神经内科和重庆医科大学神经生物学研究室。材料:实验于2003-04/2003-10在重庆医科大学生物学实验室完成。选择健康Wistar大鼠120只,雄性,体质量250～300g,由重庆医科大学实验动物中心提供。方法:实验分为4部分,每部分(n=30)均随机分为对照组(n=5)和手术组(n=25),并将手术组进一步分为出血后6h,1d,3d,5d,7d共5个时相组(n=5)。手术组大鼠麻醉后将定量胶原酶注入脑左侧尾状核建立脑出血模型;对照组只进针不注胶原酶。造模成功标准:将大鼠尾巴提起,瘫痪侧前肢回收后屈曲于腹下,正常侧前肢向地面伸展。实验分为以下4个部分:①采用免疫组化检测水通道蛋白4蛋白的表达。②采用原位杂交法检测水通道蛋白4mRNA的表达。③采用反转录-聚合酶链反应检测水通道蛋白4mRNA的表达。④应用电镜观察大鼠脑水肿的病理改变。主要观察指标:①各组大鼠水通道蛋白4mRNA及其蛋白的表达水平。②造模大鼠脑水肿区的病理改变。结果:手术组大鼠均造模成功,全部大鼠均进入结果分析,无脱失。①各组大鼠水通道蛋白4mRNA及其蛋白的表达水平比较:对照组大鼠水通道蛋白4的表达无明显改变。与对照组相比,手术组大鼠脑出血后6h,水通道蛋白4mRNA和蛋白在脑水肿区表达增强;至第3天,水通道蛋白4mRNA和蛋白的表达达到高峰;1周后,水通道蛋白4的表达仍显著高于对照组(t=12.65,P<0.01)。②脑水肿形成过程中水通道蛋白4mRNA和蛋白的关系:水通道蛋白4mRNA和蛋白的表达呈高度正相关(r=0.8281～0.9821,P<0.01)。③手术组大鼠脑水肿区�BACKGROUND： Aquaporin-4 may be one of the candidates for inducing the brain edema in ischemic stroke, however, it still has not been reported whether aquaporin-4 is involved in the formation of brain edema after intracerebral hemorrhage （ICH）. OBJECTIVE： To observe the expression of aquaporin-4 of cerebral tissue in the pathologic course of hemorrhagic cerebral edema in rats, and investigate its relationship with the formation of brain edema following ICH. DESIGN： A randomized controlled experimental study. SETTING： Department of Neurology, People＇s Hospital of Guangxi Zhuang Autonomous Region; Research Room of Neurobiology, Chongqing University of Medical Sciences. MATERIALS： This experiment was carried out in the Research Room of Neurobiology, Chongqing University of Medical Sciences between April and October 2003. Totally 120 adult healthy male Wistar rats, weighing 250-300 g, were provided by the Experimental Animal Center of Chongqing University of Medical Sciences. METHODS： The experiment had four parts, each part （n=30） was divided into the control group （n=5） and operation group （n=25）, and then the latter was subdivided into 6-hour, 1, 3, 5 and 7-day following ICH groups with 5 rats in each group. In the operation group, ICH models were established by infusing collagenase into left caudate nucleus of rats unilaterally. The operative process in the control group was the same as that in the operation group except for infusing collagenase. Standards for successful model establishment： the paralytic forelimb flexed under abdomen after withdrawal, and the normal forelimb extended to the ground. ① The expression of aquaporin-4 was detected with immunohistochemistry. ② The expression of aquaporin-4 mRNA was assessed with in situ hybridizution. ③ The expression of aquaporin-4 mRNA was assessed with reverse transcription polymerase chain reaction （RT-PCR）.④The pathological changes of hemorrhagic edema were observed under transmission electron microscope. MAI

关 键 词：脑出血 脑水肿 水孔蛋白类 

分 类 号：R743[医药卫生—神经病学与精神病学]