Ran HLA-A2.1限制性CTL表位的预测及初步鉴定  

Prediction and identification of HLA-A2.1 restricted CTL epitope from Ran

作　　者：李凡[1] 姜曼[1] 杨玓[1] 邓一静[1] 倪兵[1] 吴玉章[1] 

机构地区：[1]第三军医大学基础医学部全军免疫学研究所,重庆400038

出　　处：《免疫学杂志》2006年第3期273-276,共4页Immunological Journal

基　　金：国家自然科学基金重大项目(30490240);国家杰出青年科学基金(30325020);国家自然科学基金面上项目(30571835)资助

摘　　要：目的寻找并鉴定肿瘤相关抗原Ran来源的HLA-A2.1限制性CTL表位,为临床开展基于Ran表位的特异性免疫治疗奠定基础。方法应用超基序和量化基序相结合的方法初步预测Ran的HLA-A2.1限制性CTL表位,利用其与T2细胞亲和力实验以及与T2细胞结合稳定性试验初步验证预测结果。结果应用超基序和量化基序相结合的方法得到4个候选表位IMFDVTSRV(88-96),TLGVEVHPL(42-50),YVATLGVEV(39-47)和VLCGNKVDI(118-126),亲和力试验结果显示IMFDVTSRV,TL-GVEVHPL和YVATLGVEV有较高的的亲和力而VLCGNKVDI无明显亲和力,结合稳定性试验显示在这4个候选肽中IMFD-VTSRV的结合稳定性最好。结论IMFDVTSRV最有可能是肿瘤抗原Ran的HLA-A2.1限制性CTL表位。Objective To isolate HLA-A2.1 restricted epitopes from a new tumor associated antigen, Ras-like nuclear protein （Ran）, for application of specific immunotherapy based on Ran-derived epitope. Methods Supermotif and quantitative motif methods were used in prediction of HLA-A2.1 restricted cytotoxic T lymphocyte epitope from Ran antigen. Then the prediction was evaluated by T2 binding test, Results Four candidate epitopes were predicted by supermotif combined with quantitative motif. Four synthetic nonameric peptides were above 90% in purity and the determined values of molecular weight conformed to their theoretical values. Among four predicted epitopes, IMFDVTSRV, TLGVEVHPL, and YVATLGVEV were determined as the peptides with high IlL A-A2.1 affinity. In addition, IMFDVTSRV showed best stability in DC50 test. Conclsion The epitope prediction and peptide binding assay show that IMFDVTSRV may be the HLA-A2.1 restricted epitope from Ran.

关 键 词：RAN 肿瘤相关抗原 细胞毒性T淋巴细胞 表位 

分 类 号：R730.3[医药卫生—肿瘤]