白细胞介素-6诱导相关新基因LX3的细胞定位研究  

Localization of novel gene LX3 expression induced by IL-6 in cultivated cells

作　　者：夏荣[1] 黎燕[2] 林周[2] 沈倍奋[2] 

机构地区：[1]南方医科大学南方医院输血科,广州510515 [2]军事医学科学院基础医学研究所,北京100850

出　　处：《免疫学杂志》2006年第3期277-279,共3页Immunological Journal

基　　金：国家杰出青年科学基金(39925019)

摘　　要：目的研究IL-6诱导相关新基因LX3在真核细胞内的表达定位。方法构建真核融合表达载体pEGFP-N1/LX3,运用Lipofectin转染293T和NIH3T3细胞。共聚焦荧光显微镜检测GFP荧光,以确定IL-6诱导相关新基因LX3在细胞内的表达定位情况。结果获得真核表达载体pEGFP-N1/LX3,并成功转染293T和NIH3T3细胞,共聚焦荧光显微镜观察了LX3-GFP在细胞内的荧光分布。结论IL-6诱导相关新基因在细胞内为全细胞分布。Objective To analyze the expressional localization of novel gene LX3 induced by IL-6. Methods The LX3 eDNA was cloned into the eukaryotie expression vector pEGFP-N1, which containing green fluorescence protein （GFP） gene, and then the recombinant was transfected into 293T cell and NIH3T3 cell by Lipofection. The LX3-GFP fusion protein was detected by laser eonfocal microscopy. Results The eukaryotic expression vector pEGFP-N1-LX3 was constructed successfully; the LX3 gene was transfected into 293T cell and NIH3T3 cell by Lipofection. The location of LX3 expression in cultivated cells was detected by laser confocal microscopy. Conclusion LX3 gene proves to be expressed in the whole cell.

关 键 词：IL-6 新基因 真核表达 细胞定位 

分 类 号：R392[医药卫生—免疫学]