^(32)P胶体小鼠种植肝癌内注射后骨骼放射性的研究  

Radioactive study in bones after injection colloidal ^(32)P into implanted hepatic carcinoma of mice

作　　者：叶远红[1] 杨崇美[1] 刘吉勇[1] 李红旗[2] 

机构地区：[1]山东省立医院内科,山东济南250021 [2]山东省医学影像学研究所,山东济南250021

出　　处：《医学影像学杂志》2006年第4期353-355,共3页Journal of Medical Imaging

摘　　要：目的:研究肿瘤内单纯注射32P胶体和先注射聚合白蛋白(MAA),再注射32P胶体两种给药方法的骨骼内的放射性动态分布,探讨不同剂量MAA对32P扩散的阻滞效果。方法:在Balb/c小鼠右侧胸前皮下接种H22肝癌细胞,10天后接种部位长出直径约1cm的肿瘤。随机将动物模型小鼠分为4组:A组只注射32P胶体1.85MBq;B组先注射1×104颗粒MAA,再注射32P胶体1.85MBq;C组先注射1×105颗粒MAA,再注射32P胶体1.85MBq;D组先注射1×105颗粒MAA,再注射32P胶体18.5MBq。注射后30min、24h、48h、第8天和第16天时处死小鼠,测定骨骼的放射性。结果:瘤内局部注射32P胶体时,骨骼内可检测到32P的放射性,当向肿瘤内注射的32P胶体前,预先注入MAA组小鼠,其骨骼32P分布均比未注射MAA的一组明显少,其中1×105颗粒MAA组的小鼠,32P骨骼的分布又比1×104颗粒MAA组少;当预先注入的MAA颗粒数量相同时,注射的32P胶体剂量加,骨骼分布亦随之增加。结论:与单纯瘤内注射32P胶体相比,先在瘤内注入MAA,再注入32P胶体,MAA可以有效阻止32P胶体的扩散,减少了32P骨骼分布,从而减少骨髓抑制副作用的发生率。Objective：To study dynamic distribution of colloidal ^32 p of tumoral tissues and bones in single colloidal ^32 p injection and injection of MAA followed by colloidal ^32 P,To observe retardent effect of MAA.Methods： Balb/c mices were inoculated subcutaneously by H22 hepatocellular cancer cells in the right fore leg. When the tumors reach 1.0 cm of diameter, about 10 days postinjection, The mices were divided into four groups randomly . A groups ： the tumors were only injected slowly with 1.85Mq colloidal ^32 P; B groups： 1 × 10^4 particles of MAA followed by 1.85MBq colloidal ^32 p; C group： 1 × 10^5 particles of MAA followed by 1.85MBq colloidal ^32 p; D group： 1 × 10^5 particles of MAA followed by 18.5MBq colloidal ^32 p. the mices of each group were sacrificed at 30min ,24h , 48h,8d and 16d postinjection. Tumoral tissues and bone of each animal were counted for radioactivity in a garmna counter. Results： Radioactivity of bone tissuse was detected if we inject ^32 p into the center of tumors, when the dose of ^32 p colloidal injected into tumor was determined, the distribution of ^32 p bones in injected MAA groups in advance was lower than than in only injected colloidal ^32 p group, but the distribution of^32 P in 1 × 10^5group was lower than that in 1 × 10^4 group; when the quantity of MAA particles was determined, distribution of^32 P increased following the increase of injected intratumoral colloidal ^32 p. Conclusion： If we inject MAA into the center of tumors in advance, then inject colloidal ^32 p, MAA could retard diffusion of colloidal ^32 p from tumor tissuse to the bones, therefore decrease the distribution of bones, decrease the side effect of inhibition of bone marrow.

关 键 词：肝肿瘤 治疗 核素 聚合白蛋白 小鼠 

分 类 号：R735.7[医药卫生—肿瘤] R817[医药卫生—临床医学]