原位异种移植性骨肉瘤的动物模型及生物学特性的初步观察  

A model of rat osteosarcoma in nude mice using orthotopic implantation and studies on its biological characteristics

作　　者：李艳[1] 向理科[2] 罗娟[3] 王智彪[4] 

机构地区：[1]重庆医科大学附属儿童医院病理科,重庆400014 [2]重庆医科大学病理学教研室,重庆400016 [3]浙江省肿瘤医院病理科,杭州310022 [4]重庆医科大学生物医学工程系,重庆400016

出　　处：《重庆医科大学学报》2006年第2期137-140,共4页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(30171060)

摘　　要：目的:建立大鼠UMR106骨肉瘤细胞株的裸鼠原位移植模型。方法:采用完整瘤组织块原位移植到裸鼠右胫骨骺端,分不同时期处死,观察其生长及转移特性。结果:肿瘤原位移植成功率为100%,移植后1￣2w从髓内向髓外生长,3w已侵犯髓外软组织并有明显骨皮质破坏,4￣5w有肺转移,6w左右裸鼠出现恶病质,且双肺广泛转移,最终全身衰竭死亡。结论:该模型成功率高,模拟了人类骨肉瘤临床发病和发展过程,可作为研究骨肉瘤较理想的模型。Objective： To establish a model of rat osteosarcoma in nude mice and to study its biological characteristics. Methods： Intact tumor pieces were inoculated into the right tibia of nude mice. The nude mice were killed at different time and the growth of tumor and metastasis were observed. Result： The tumor growth rate was 100%. From the first week to the second week after implantation it grew into outer tissue from tibia, and widely invaded Soft tissue on the third week. Pulmonary metastasis took place from the fourth week to the fifth week, and the nude mice died of cachexia and respiratory failure resulting from the widely whole pulmonary metastasis on the sixth week or so. Conclusion： The tumor growth rate is high. The model simulates the clinical process of human osteosarcoma, and could be a satisfactory model for studying osteosareoma.

关 键 词：骨肉瘤 裸鼠 原位移植 

分 类 号：R730.59[医药卫生—肿瘤]