鼻咽癌病人EB病毒EBNA-2A及EBNA-LP IgG和IgA抗体的测定  

DETECTION OF IgG AND IgA ANTIBODIES TO EPSTEIN-BARR VIRUS EBNA-2A AND E8NA-LP IN SERA FROM PATIENTS WITH NA30PHA-RyNGEAL CARCINOMA AND FROM NORMAL INDIVIDUALS

作　　者：韩汝晶 F.Huang E.Kieff 曾毅[3] 

机构地区：[1]吉林医学院 [2]美国哈佛大学医学院微生物室 [3]中国预防医学科学院病毒学研究所

出　　处：《病毒学报》1990年第3期228-232,共5页Chinese Journal of Virology

摘　　要：Epstein-Barr病毒核抗原(EBNA)是EB病毒潜伏感染时表达的主要抗原之一。它与EB病毒潜伏感染和转化正常细胞引起肿瘤有关。本实验用间接免疫荧光法(IIF),采用经EB病毒表达EBNA-2A及EBNA-LP的基因组转染的大鼠成纤维细胞作为靶细胞,测定了30例鼻咽癌(NPC)病人和30例正常人血清中的EBNA-2A及EBNA-LP的IgG和IgA抗体,结果表明,30例NPC病人的IgG/EBNA-2A抗体阳性率为100％,平均几何滴度(GMT)为1:38.9。而30例正常人的阳性率为70％,GMT为1:7.9。两者有显著性差异。在30例NPC病人IgG/EBNA-LP抗体的阳性率为87％,GMT为1:9.3。30例正常人的阳性率为67％,GMT为1:7.9。两者之间无明显差异。IgA/EBNA-2A和IgA/EBNA-LP抗体在NPC病人和正常人均为阴性。这可能是由于在NPC病人癌细胞中没有EBNA-2A和EBNA-LP这两种抗原的表达。这结果对进一步研究EB病毒与NPC发生的关系有一定意义。The Epstein-Barr virus nuclear antigen ( EBNA)is one of the main antigen expressed in latent infection. It is a component of different proteins. IgG and IgA antibodiss to EBNA-2A and EBNA-LP were detected in sera from NPC patients and from normal individuals by the indirect immuno-fluorescence test. Cells transfected with EBV genes expressing EBNA-2A or EBNA-LP were used as target cells. For IgG/EBNA-2A antibody 100% of NPC patients were positive with GMT 1 : 38.9 and 70% of normal individuals were positive with GMT 1:7.9 The positive rate and GMT of these two groups were significantly different. For IgG/EBNA-LP antibody. 87% of NPC patients were positive with GMT 1 :9.3 and 67% normal individuals were positive with GMT 1: 7.9. After removal of IgG antibodies with SPA, IgA/EBNA-2A and IgA/EBNA-LP were all negative in both NPC patients and in normal individuals. The result perhaps is due to non expression of EBNA-2A and EBNA-LP in NPC cancer cells.

关 键 词：鼻咽肿瘤 EB病毒 核抗原 

分 类 号：R739.630.3[医药卫生—肿瘤]