延迟性低氧预适应降低小鼠脑皮质cPKCγ蛋白表达  

Delayed hypoxic preconditioning decreases the level of cPKCγ protein expression in cerebral cortex of mice

作　　者：韩松[1] 牛晨晨[1] 李俊发[1] 

机构地区：[1]首都医科大学基础医学院神经生物学系,北京100054

出　　处：《基础医学与临床》2006年第4期372-375,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金(30270508;30470650);北京市自然科学基金(7032005);教育部优秀青年教师资助项目;北京市教委科技发展计划项目(KM200310025100)

摘　　要：目的探讨cPKCα和γ膜转位水平及蛋白表达量在延迟性脑低氧预适应形成过程中的变化。方法应用SDS-PAGE和Western bolt等技术,并结合Gel Doc凝胶成像系统,半定量检测延迟性脑低氧预适应小鼠(H5和H6组)脑组织内cPKCα和γ的膜转位水平及蛋白表达量。结果延迟性低氧预适应(H5和H6组)明显降低小鼠脑皮质组织内cPKCγ蛋白表达量(P<0.05),而膜转位(活性)水平变化不显著。对海马cPKCγ、大脑皮质和海马cPKCα蛋白表达量及膜转位水平的影响均不显著。结论大脑皮质cPKCγ蛋白表达量的改变可能参与延迟性脑低氧预适应形成,且可能与皮质和海马低氧选择易损性的差异有关。Objective To explore the role of conventional protein kinase C （cPKCs） in delayed hypoxic preconditioning. Methods The biochemistry techniques of SDS-PAGE, Western bolt and Gel Doc imagine were applied to analyze the effect of repetitive hypoxic exposure （H5, H6） on the level of cPKCα, T membrane translocation and protein expression in murine brain. Results We found that cPKCT protein expression was significantly decreased （ P 〈 0.05, n = 6） as a response to delayed hypoxic preconditioning in cortex [H0：100% vs H5- H6：（69.2 ± 7.6） %, （76.1 ±7.3） % ]. But there was no significant change in the level of cPKCγ protein expression in hippocampi, cPKCα prortein expression level and cPKCα, T membrane translocation both in cortex and hippocampi. Conclusion The changes of cPKCγ protein expression is only involved in late phase of cerebral hypoxic preconditioning. In addition, profile of cPKCγ protein expression both in cortex and hippocampus may be related to their selective vulnerability to hypoxia.

关 键 词：脑低氧预适应 经典型蛋白激酶C 膜转位 蛋白表达量 脑组织 

分 类 号：R363[医药卫生—病理学]