JWA在N-甲基-N’-硝基-N-亚硝基胍诱导的人支气管上皮细胞凋亡中的作用  

The role of JWA in N-methyl-N'-nitro-N-nitrosoguanidine induced human bronchial epithelial cell apoptosis

作　　者：徐艳琼[1] 李爱萍[1] 陈瑞[1] 周建伟[1] 

机构地区：[1]南京医科大学公共卫生学院江苏省人类功能基因组学和应用毒理学重点实验室分子毒理研究室,210029

出　　处：《中华劳动卫生职业病杂志》2006年第4期205-208,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金(30471430);"973"国家重大基础研究规划项目基金(2002CB512900)

摘　　要：目的探讨JWA在化学致突变物烷化剂N-甲基-N’-硝基-N-亚硝基胍(MNNG)诱导人支气管上皮(HBE)细胞凋亡中的作用及其可能机制。方法用噻唑蓝(MTT)法检测细胞生长抑制率,用Hoechst染色法检测细胞凋亡,用Western blot法检测JWA蛋白表达,用Southwestern印迹分析法检测JWA基因近端启动子的结合蛋白。结果MNNG处理HBE细胞24 h均可诱导细胞发生凋亡,并呈现明显的剂量-效应关系；MNNG诱导HBE细胞凋亡过程中伴随着JWA蛋白表达升高。进一步用2．0μg/ml MNNG处理HBE细胞24h后,用Southwestern方法从HBE细胞核蛋白中检测出一与JWA近端启动子能特异性结合的转录因子。结论MNNG激活HBE细胞中核转录因子与JWA近端启动子结合后可能激活了细胞内凋亡的信号通路。Objective To investigate the role and possible mechanism of JWA in N-methyl-N＇-nitro-N-nitrosoguanidine（MNNG） induced human bronchial epithelial（HBE） cell apoptosis. Methods The ceil growth inhibition rate was detected by MTr, the cell apoptosis was measured by Hoechst staining, the expression of JWA protein was detected by Western blot, and the potential binding protein of JWA proximal promoter was detected by Southwestern assay. Results MNNG treatment of HBE ceils for 24 hours induced apoptosis with significant doseeffect relationship and in this course the expression of JWA protein was elevated. The 2.0 μg/ml MNNG treated cells for 24 hours activated nuclear transcription factor expression that specifically bound to JWA proximal promoter. Conclusion That MNNG treatment activates nuclear transcription factor binding to JWA proximal promoter may be involved in intraceilular apoptosis associated signal pathway.

关 键 词：基因 JWA 细胞凋亡 烷化剂 转录因子 

分 类 号：R363[医药卫生—病理学]