人卵巢上皮癌的体外培养及耐药株的诱导  

In vitro culture of human epithelial ovarian carcinoma and establishment of drug-resistance cell line.

作　　者：梁慧超[1] 王沂峰[1] 

机构地区：[1]广州医学院附属市二人民医院妇产科,510950

出　　处：《广东医学》2006年第5期642-644,共3页Guangdong Medical Journal

基　　金：广东省社会发展领域科技计划项目(编号:53);广东省名医工程研究项目(编号:30);广州市医药卫生科技项目(编号:03402)

摘　　要：目的研究人卵巢上皮癌体外培养的方法及其耐药株的诱导方法。方法取卵巢上皮癌患者的卵巢癌实体瘤、囊内液及腹水,分别用直接培养法,胰酶消化培养法和胶原酶消化培养法体外培养。检测细胞对顺铂和环磷酰胺的药物敏感性,选择适当的药物和浓度诱导卵巢上皮癌耐药株。并比较耐药株与诱导前的母株间克隆形成率和耐药蛋白表达的差异。结果腹水和囊内液直接培养较实体瘤培养易成功。实体瘤培养以胶原酶消化培养法获得细胞数最多,混杂细胞最少。取腹水来源的稳定传至30代的卵巢癌细胞用环磷酰胺诱导耐药株成功。检测其克隆形成率和耐药蛋白表达均高于诱导前。结论本实验探讨了人卵巢上皮癌体外培养的多种方法,成功培养一株卵巢黏液性囊腺癌细胞,达建系要求,并成功诱导其耐药株。可用于卵巢上皮癌发生及复发机制和治疗及预防方面的研究。Objective To study different methods of in vitro culture of human epithelial ovarian carcinoma, and the method of inducing drug - resistance cell line it, Methods Tissue of humen epithelial ovarian carcinoma was cultured in vitro after mechanical dissociation, trypsin dissociation, or collagenase dissociation. And the fluid of cyst or malignant ascites were directly cultured, Clonogenic assays - ehemosensitivity tests were performed to select suitable medicine and the concentration to induce drug- resistant cell line. Clonogenie assay and the expression of ABCG2 between drug- resistant cells and original cells were compared. Results Direct culture of the fluid of cyst or malignant ascites was easier to succeed. Concerning the solid tumor in vitro, the collagenase dissociation gain more single cells and less contaminated cells. The drug - resistant cell llne was successfully by clophosphamide induced from the thirtieth subline of ovarian cancer originated from ascites. The clonogenic ability and the expression of ABCG2 of the drug- resistant cells were higher than those of original cells. Conclusion Different methods of culturing ovarian cancer cell in vitro, and inducing drug - resistant cells were tested in this study. An ovarian cancer cell line and drug - resistant cell line suecessfully were cultured in vitro fully, It may contribute to the improvement of our knowledge of the patho- genesis and the treatment of humam ovarian cancer.

关 键 词：卵巢肿瘤 体外培养 耐药性 

分 类 号：R730.5[医药卫生—肿瘤] R329-33[医药卫生—临床医学]