复方黄芪提取物对细菌脂多糖诱导大鼠腹腔巨噬细胞分泌TNFα、NO及IL-1的影响  

Effect of compound astragalus extract on TNFα、NO and IL-1 secreted by rat peritoneal macrophages induced by lipopolysaccharide

作　　者：路景涛[1] 陈敏珠[1] 

机构地区：[1]安徽医科大学临床药理研究所,安徽合肥230032

出　　处：《安徽医药》2006年第5期330-331,共2页Anhui Medical and Pharmaceutical Journal

摘　　要：目的 研究复方黄芪提取物（CAE）对细菌脂多糖诱导大鼠腹腔巨噬细胞（PMφS）释放肿瘤坏死因子（（TNFα）、白细胞介素-1（IL-1）及一氧化氮（NO）的影响。方法 10mg／L LPS体外诱导大鼠PMφS分泌TNFα、IL-1与NO等因子，采用小鼠成纤维细胞瘤株（L929）杀伤法检测TNFα的含量、小鼠胸腺细胞增殖法检测IL-1的活性、Griess法检测NO的水平。结果 CAE（11．3-90mg·L^-1）能显著抑制LPS激活的巨噬细胞对TNFα、IL-1与NO的分泌。结论 CAE抑制巨噬细胞的分泌功能可能是其保肝作用的机制之一。Aim To study effect of compound astragalus extract （CAE）on TNFα, IL-1 and NO secreted by normal rat peritoneal macrophages （PMφ）. Methods Rat peritoneal macrophages was stimulated with 10 mg · L^-1 LPS in vitro. TNFα was measured by assay of cytotoxicity against L929 cells ,The concentration of NO was determined by Griess assay, activity of IL-1 was assayed by [ ^3H ] TdR incorporation assay. Results CAE could markedly inhibit production of TNFα, IL-1 and NO from normal rats PMφS induced by LPS. Conclusion Inhibiting directly secretion of TNFα, IL-1 and NO produced by PMφS might be one of the mechanisms of hepatoproteetive effect of CAE.

关 键 词：CAE PMΦ TNFΑ IL-1 NO 

分 类 号：R285[医药卫生—中药学]