Fabrication, in vitro Degradation and Cytotoxic Assay of Different Cystalline Phases Calcium Polyphosphate  

作　　者：邱凯 万昌秀 

机构地区：[1]Department of Polymer Science and Engineering, Sichuan University, Chengdu 610065, China

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2005年第B12期111-113,共3页武汉理工大学学报（材料科学英文版）

基　　金：FundedbytheNationalNaturalScienceFoundationofChina(50472091)

摘　　要：Fabrication, in vitro degradation and cytotoxic assay of different crystalline phases calcium polyphosphate（CPP） were reported. The CPP ceramics were fabricated by crystallizing the amorptous frits, and sintered at 550℃ ,875℃, 1000℃ for 1 h to obtain the 7-CPP, β-CPP and α-CPP respectively. The efffects of the different crystalline phases on their weight loss and released PO4^3- were investigated during the degradation. And the surface change was observed by the SEM, The osteoblastic ROS17/2.8 cell line was used to estimate the cytotoxicity of CPP. The effects of CPP on cells ＇ proliferation were evaluated by using MTT assay. The experimental restdts showed that γ-CPP, β-CPP and α-CPP did not exert cytotoxic effect on the cells. In addition, the proliferation of the growth of ROS17/2.8 cells on β-CPP was optimal.Fabrication, in vitro degradation and cytotoxic assay of different crystalline phases calcium polyphosphate（CPP） were reported. The CPP ceramics were fabricated by crystallizing the amorptous frits, and sintered at 550℃ ,875℃, 1000℃ for 1 h to obtain the 7-CPP, β-CPP and α-CPP respectively. The efffects of the different crystalline phases on their weight loss and released PO4^3- were investigated during the degradation. And the surface change was observed by the SEM, The osteoblastic ROS17/2.8 cell line was used to estimate the cytotoxicity of CPP. The effects of CPP on cells ＇ proliferation were evaluated by using MTT assay. The experimental restdts showed that γ-CPP, β-CPP and α-CPP did not exert cytotoxic effect on the cells. In addition, the proliferation of the growth of ROS17/2.8 cells on β-CPP was optimal.

关 键 词：calcium polyphosphate DEGRADATION CYTOTOXICITY MTT 

分 类 号：R318.08[医药卫生—生物医学工程]