Effects of β-Tricalcium Phosphate on the Growth of Rat Osteoblasts  

作　　者：袁琳 

机构地区：[1]Biomedical Materials and Engineering Research Center, Wuhan University of Technology, Wuhan 430070, China

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2005年第B12期187-189,共3页武汉理工大学学报（材料科学英文版）

基　　金：FundedbytheScientificResearchFoundationfortheReturnedOverseasChineseScholars,StateEducationMinistryandWuhanUniversityofTechnology.

摘　　要：Calcium-phosphate materials, such as β- TCP could be degraded in vivo , and in the cultured cells in vitro. However , it is still unknown how the materials changed the activity of the cells. In order to understand the mechanism of β- TCP treatment on osteoblasts , cell proliferation rate measurement and microscopic observation were performed during co-culture of rat osteoblasts and β- TCP biomaterials. It was observed that low concentrations of β- TCP-treated cell proliferation rate was a little less than control by MTT assay, while higher concentratioras of β- TCP-treated cell proliferation rate was more than control. The proliferation rate of osteoblast treated by β-TCP with bioglass had a similar effect with β-TCP-treated cell. Neither calcium chloride nor sodium monophosphate treatment could stimulate the cell proliferation at the concentration from 0.25 mM to 5 mM. During the treatment of β- TCP , particles could be found in the cells. It suggested that β- TCP could stimulate cell proliferation rate of rat osteoblast with or without bioglass , while Ca^2＋ and PO4^3＋（ provided by calcium chloride and sodium monophosphate , separately）, the degraded products of β- TCP had no significant effect on osteoblast cell growth in a wide range. The stimulating effect of β- TCP on osteoblast cell proliferation might relate to the encapsulation of the ruclterial particles into the cells.Calcium-phosphate materials, such as β- TCP could be degraded in vivo , and in the cultured cells in vitro. However , it is still unknown how the materials changed the activity of the cells. In order to understand the mechanism of β- TCP treatment on osteoblasts , cell proliferation rate measurement and microscopic observation were performed during co-culture of rat osteoblasts and β- TCP biomaterials. It was observed that low concentrations of β- TCP-treated cell proliferation rate was a little less than control by MTT assay, while higher concentratioras of β- TCP-treated cell proliferation rate was more than control. The proliferation rate of osteoblast treated by β-TCP with bioglass had a similar effect with β-TCP-treated cell. Neither calcium chloride nor sodium monophosphate treatment could stimulate the cell proliferation at the concentration from 0.25 mM to 5 mM. During the treatment of β- TCP , particles could be found in the cells. It suggested that β- TCP could stimulate cell proliferation rate of rat osteoblast with or without bioglass , while Ca^2＋ and PO4^3＋（ provided by calcium chloride and sodium monophosphate , separately）, the degraded products of β- TCP had no significant effect on osteoblast cell growth in a wide range. The stimulating effect of β- TCP on osteoblast cell proliferation might relate to the encapsulation of the ruclterial particles into the cells.

关 键 词：Β-TCP OSTEOBLAST cell proliferation 

分 类 号：Q813.11[生物学—生物工程]