人外周血淋巴细胞及单核细胞表达PD-L1和PD-L2的动力学研究  

Kinetic expressions of PD-L1 and PD-L2 on the surface of human lymphocytes and monocytes

作　　者：迟晓云[1] 何贤辉[1] 查庆兵[1] 徐丽慧[1] 曾耀英[1] 王通[1] 

机构地区：[1]暨南大学组织移植与免疫教育部重点实验室

出　　处：《中国病理生理杂志》2006年第5期873-877,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金重点项目(No.30230350);国家自然科学基金面上项目(No.30371651);国家重点基础研究发展规划项目(No.G200057006)

摘　　要：目的:探讨PD-L1和PD-L2在正常人外周血静息和活化的B细胞、T细胞及单核细胞表面的表达规律。方法:利用荧光抗体染色和流式细胞术分析静息状态及经多克隆刺激剂脂多糖(LPS)和美洲商陆丝裂原(PWM)刺激6、24、48和72 h后表达PD-L1和PD-L2的B细胞和T细胞百分率,同时分析静息状态及经IFN-γ和LPS共同刺激244、8、72和96 h后表达PD-L2的单核细胞百分率。结果:静息B细胞和T细胞表面不表达PD-L1,LPS和PWM刺激6 h后表达PD-L1的B细胞百分率均显著高于静息B细胞,24 h达到最高,分别为(46.26±10.71)%和(43.67±6.14)%,之后随时间延长而降低;表达PD-L1的T细胞百分率在LPS刺激下无显著变化,但PWM刺激6 h后百分率明显高于静息条件,24 h达到最高,为(25.42±9.23)%,之后下降。静息B细胞和T细胞不表达PD-L2,活化后PD-L2表达也无明显上调。另外,静息状态单核细胞表面不表达PD-L2,体外活化24 h后表达PD-L2的单核细胞百分率显著上调,48 h达到最高为(28.70±14.22)%,之后随时间而降低。结论:活化的淋巴细胞表达PD-L1,但不表达PD-L2,后者在活化的单核细胞表面表达,并且后者达到高峰的时间迟于前者。AIM： To investigate the expression kinetics of PD- L1 and PD- L2 on the surface of the resting and activated B/r cells as wen as monocytes from healthy human peripheral blood. METHODS： Fluorescent antibody staining together with flow cytometry were used to detect the percentages of the resting as well as the activated B ccl.ls and T cells that expressed PD-L1 and PD- 1.2. Meanwhile the percentages of the resting and activated monocytcs that expressed PD - L2 were determined. SULTS： Both resting B cells and T cells did not express PD- LI on their surface, however PD- L1 expression was significantly up - regulated on the surface of the activated B cells after 6 h stimulation with LPS or pokeweed mitegen （PWM）, and the percentages of B cells that expressed PD - L1 reached a plateau at 24 h, which were （ 46.26 ± 10.71 ） % with LPS and （43.67 ± 6.14） % with PWM stimulation, respectively. No markedly change of PD- L1 expression on the surface of the activated T cells after stimulation with LPS was observed, but upregulation of PD - L1 expression was observed when stimulation with PWM. The percentages of T cells that expressed PD- L1 reached a plateau at 24 h, which was （25.42 ± 9.23）%. PD- L2 expression was not found on the resting as well as the activated B cells and T cells. In addition, the resting monocytes did not express PD - L2. Combination of INF - γ plus LFS markedly induced the PD- L2 expression, and the percentages of monocytes that expressed PD- L2 reached a peak at 48 h, which was （28.70± 14.22）%. CONCLUSION： The activated lymphoeytes only express PD- L1, reaching a plateau at 24 h. PD-L2 is expressed on the surface of the activated monocytes, roaching a peak at 48 h.

关 键 词：B7-H1 B7-DC 脂多糖类 商陆有丝分裂原类 流式细胞术 淋巴细胞 单核细胞 

分 类 号：R392.12[医药卫生—免疫学]