激活素受体样激酶1促进人脐静脉内皮细胞增殖和迁徙  

The activin receptor-like kinase Ⅰ promotes proliferation and migration in HUVECs

作　　者：李斌[1] 唐仕波[1] 林少芬[1] 孟晶[1] 

机构地区：[1]中山大学中山眼科中心,广东广州510060

出　　处：《中国病理生理杂志》2006年第5期904-907,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金群体创新项目资助(No.30321004);国家自然科学基金资助项目(No.30471849);广东省重点科技项目基金资助(No.052001144A)

摘　　要：目的:研究激活素受体样激酶1(ALK1)对人脐静脉内皮细胞的作用。方法:体外培养人脐静脉内皮细胞(HUVECs),RT-PCR分析ALK1和ALK5在HUVEC激活状态下表达的变化。脂质体转染pcDNA3.1+ALK1到HUVECs,流式细胞仪检测HUVECs增殖的改变,boyden小室检测ALK1对HUVECs迁徙的影响。结果:ALK1在HU-VEC安静状态高表达,ALK1能促进HUVECs的增殖和迁徙。结论:ALK1通过促进内皮细胞的增殖和迁徙在血管重塑中发挥作用。AIM： To study the function of activin receptor- like kinase Ⅰ （ALK1） gene in vascular endothelium. METHODS： The human umbilical vein endothelial cells （HU3/ECs） were cultured, and the change of expression of ALK1, in activation of HUVECs was analyzed. The full - leng coding sequence of ALK1 was cloned into peDN/L3.1 ＋ using standard protocols. The construeted pcDNA3.1 ＋ ALK1 plasmid were transfeeted into HUVECs. The proliferation and migration of HUVECs were detected by boyden champer and flow cytometry. RESULTS： The expression of ALK1 was up- regulated in resolution. ALK1 prorooted the proliferation and migration of HUVECs. CONCLUSION： ALK1 has an important function in remodeling by promoting the proliferation and migration of endothelial cells.

关 键 词：激活素受体Ⅰ 激活素受体Ⅴ 脐静脉内皮细胞 细胞增殖 细胞运动 

分 类 号：R363[医药卫生—病理学]