半边旗提取物5F诱导人翼状胬肉成纤维细胞凋亡的研究  

Effect of diterpenoid compound 5F of Peris semipinnata L on apoptosis of human pterygium body fibroblasts in vitro

作　　者：陈毓东[1] 王映芬[2] 陈建苏[1] 李志杰[1] 陈小琳[3] 

机构地区：[1]暨南大学第一临床学院眼科医学院眼科实验室 [2]广东医学院附属医院眼科,广东湛江524001 [3]暨南大学医学院病理生理实验室机能实验室,广东广州510632

出　　处：《中国病理生理杂志》2006年第5期1024-1027,共4页Chinese Journal of Pathophysiology

摘　　要：目的:观察中药半边旗二萜类化合提取物5F对体外培养的人翼状胬肉成纤维细胞(HPFs)周期的影响及5F诱导细胞凋亡过程超微结构的变化,初步探讨5F对HPFs作用的机制。方法:应用细胞培养技术、流式细胞术(FCM)分析5F对HPF作用不同时间其细胞周期及诱导凋亡的特征,电镜观测HPFs超微结构改变。结果:5F浓度32 mg/L始诱导HPFs凋亡,随5F作用时间延长细胞被抑制并由凋亡发展为坏死的形态结构改变。结论:在一定浓度下5F抑制HPFs增殖,诱导细胞凋亡、坏死,提示5F具潜在临床应用前景。AIM： To observe the effect of diterpenoid compound 5F of Pteris semipinnate L on apoptusis in cultured human pterygium body fibroblasts （HPFs）. METHODS： Fibroblasts collected from human pterygium body were cultured in vitro with 5F at different time point. Transmission electron microscope （TEM） was used to observe the ultrastructure changes of the HPFs. The changes of the cell cycle and apoptosis of HPFs were evaluated with flow cytometry （FCM）. RESULTS： 5F at the concentration of 32 mg/L induced HPF apoptosis at various time points. CONCLUSION： 5F induces HPFs apoptosis. Apoptotic cells are evolved to necrosis with the prolongation of the time. These findings indicate that 5F has a potential clinical value.

关 键 词：翼状胬肉 成纤维细胞 细胞凋亡 中草药 

分 类 号：R777.33[医药卫生—眼科] R363[医药卫生—临床医学]