尼美舒利抑制结肠癌细胞增殖及转移的机制  

The Mechanism of Nimesulide for Inhibiting Cell Proliferation and Metastasis of Human Colon Carcinoma

作　　者：费素娟[1] 陈光侠[1] 

机构地区：[1]徐州医学院附属医院消化科,江苏省徐州市221002

出　　处：《中国肿瘤临床》2006年第9期497-499,共3页Chinese Journal of Clinical Oncology

基　　金：江苏省普通高校自然科学研究计划基金资助(编号:01KJB320011)

摘　　要：目的:研究选择性COX2抑制剂尼美舒利(Nimesulide)对结肠癌细胞株SW1116生长的影响及其参与抑制肿瘤血管转移的可能机制。方法:采用四甲基偶氮唑蓝(MTT)比色法检测Nimesulide对结肠癌细胞增殖的抑制,流式细胞仪(FCM)分析其对细胞凋亡及细胞周期的影响,ELISA法检测其对上清液中血管内皮生长因子(VEGF)的影响。结果:MTT显示Nimesulide能呈剂量和浓度依赖性抑制SW1116的增殖;FCM显示此药促进细胞的凋亡,使处于G0/G1期的细胞比例显著降低;VEGF的含量呈时间依赖性下调。结论:Nimesulide可抑制结肠癌细胞株SW1116生长,促进其凋亡,其机制可能与其阻止细胞周期的进展有关。此外,Nimesulide还可通过下调上清中的VEGF抑制肿瘤生长及浸润转移。Objective： To study the mechanism of selective cyclooxygenase-2 （COX-2） inhibitor Nimesulide affecting growth and inhibiting metastasis of human colon cancer cells. Methods： MTT assay and flow cytometry （FCM） were used to determine the effect of Nimesulide on the proliferation and apoptosis, as well as the cell cycle of colon cancer cells. VEGF was examined in the supernatant fluid using ELISA. Results： Nimesulide inhibited the proliferation of colon cancer cells in a dose- and timedependent manner and decreased the proportion of cells in the G0/Gl phase. They also reduced the release of VEGF in a time-dependent way. Conclusions： Nimesulide can inhibit proliferation and induce apoptosis in eolorectal cancer cell lines by blocking cell cycle progression, and may inhibit proliferation and metastasis of human colorectal carcinoma by down-regulation of the release of VEGF in the supernatant.

关 键 词：结肠癌细胞 尼美舒利 血管内皮生长因子 

分 类 号：R735.35[医药卫生—肿瘤]