不同肺癌细胞RARγ受体转录水平对9-顺维甲酸诱导的反应  

Effects of 9-cis-retinoic Acid on Transcripton of Retinoic Acid Receptors RARγ in Lung Cancer Cells

作　　者：王虎[1] 于雪艳[2] 崔琪琼[1] 张士国[3] 胡国强[4] 任贵杰[2] 田克力[2] 

机构地区：[1]中国医学科学院中国协和医科大学阜外心血管病医院 [2]山东大学西校区生化教研室 [3]济南军区总医院检验科 [4]山东大学齐鲁医院胸外科

出　　处：《中国分子心脏病学杂志》2005年第3期526-529,共4页Molecular Cardiology of China

摘　　要：目的检测用9-cis-RA处理前后肺腺癌细胞株PG和A549维甲酸受体RARγ不同时相的表达变化。方法将两细胞株分别分为9-cis-RA组和对照组,应用细胞计数方法统计每组各时相细胞数,相对定量RT-PCR方法检测加药前后RARγ在不同时相的表达变化。结果加入9-cis-RA前,PG和A549细胞RARγmRNA转录水平检测结果显示RARγ在PG细胞株中有一定水平的基础转录,而在A549细胞中无基础转录。应用1uM9-cis-RA后,PG细胞的RARγmRNA的转录水平受到抑制,而A549细胞的RARγ的转录水平在各时相有较弱的诱导表达。结论RARγ受体对9-cis-RA的诱导在不同细胞株中的反应不同,表明9-cis-RA对不同肿瘤细胞株的作用存在特异性。Objective To detect expression changes of retinoid A receptor（RART） in different time- phases in PG and A549 lung glandular cancer cells before and after being treated with luM 9-cis-RArespectively. Methods Dividing PG and A549 lung glandular cancer cells each into two groups： 9-cis-RA group and control group. Cell numbers of each group in different time-phases was by cell count methods. Reverse transcription polymerase chain reaction （RT-PCR） were used in study. Results The results that RT-PCR detected for the levels of RARγ transcription in PG and A549 cells showed ： （ 1 ） There was no basic transcription of RARγin A549 cells, but show weak transcription level in PG cells; （2） After being treated with 9-cis-RA, RARγ increased weakly in A549 cells, but this transcription was inhibited in PG cells. Condusion RARγ showed different response to 9-cis-RA in PG and A549 cells.

关 键 词：9-顺维甲酸 维甲酸受体 RT-PCR 肺癌 

分 类 号：R734.2[医药卫生—肿瘤]