正畸牙齿移动过程中大鼠三叉神经节CGRP mRNA表达变化  

Changes in the expression of CGRP mRNA in the rat trigeminal ganglion during and after tooth movement

作　　者：孙应明[1] 罗颂椒[2] 

机构地区：[1]解放军第101医院口腔科,江苏无锡214044 [2]四川大学华西口腔医学院正畸科

出　　处：《临床口腔医学杂志》2006年第5期264-266,共3页Journal of Clinical Stomatology

基　　金：国家自然科学基金资助项目(30200320)

摘　　要：目的:观察正畸牙齿移动不同时期大鼠三叉神经节CGRP mRNA表达变化。方法:采用反转录-聚合酶链反应(RT-PCR)技术检测正畸加力和撤力后不同时期大鼠三叉神经节CGRP mRNA水平的变化。结果;加力后2 h CGRP mRNA的表达量没有明显增加,至加力后8 h开始增加,加力1 d^1 W达到最高峰,至撤力后2~4 WCGRP mRNA的表达量开始下降,但CGRP mRNA水平仍然明显高于对照组(P<0.05)。结论:CGRP可能不仅参与早期正畸牙周组织的炎症损伤过程,而且可能参与了后期的组织修复重建过程。Objeetive： To investigate the relationship between CGRP and orthodontic tooth movement. Method. The first maxillary molar was moved mesially by an orthodontic force for 2 hours,8 hours, 1 day,3 days, 1 week and 2 weeks, whereas the other groups were observed after removal of appliances for 1 week, 2 weeks and 4 weeks. The expression of CGRP mRNA in trigeminal ganglion were detected with reverse transcription-polymerasa chain reaction（RT-RCR）. Resuit：The expression of CGRP mRNA in trigeminal ganglion were stonger than that in control group at most parts of time points during tooth movement; CGRP mRNA level was increased after 8 hours and reached the peak level around 1 day-1 week during tooth movement, 2 weeks after tooth movement CGRP mRNA level was deereased;4 weeks after tooth movement CGRP mRNA level was still higher than that in the controls. Conclusion： The results suggested the involvement of CGRP in both early stages of periodontal remodeling and later in the regenerative processes of the periodontal ligament during and after orthodontic tooth movement.

关 键 词：降钙素基因相关肽 牙齿移动 三叉神经节 反转录-聚合酶链反应 

分 类 号：R780.1[医药卫生—口腔医学]