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作 者:任大军[1] 张晓昱[2] 颜克亮[2] 陆晓华[1]
机构地区:[1]华中科技大学环境科学与工程学院 [2]华中科技大学生命科学与技术学院,湖北武汉430074
出 处:《华中科技大学学报(自然科学版)》2006年第5期121-124,共4页Journal of Huazhong University of Science and Technology(Natural Science Edition)
基 金:教育部科学技术研究重点资助项目(104250)
摘 要:选用白腐菌BP降解吲哚,研究了白腐菌在不同培养基中对两种浓度吲哚的降解过程和机理,以及吲哚的降解与白腐菌漆酶酶活、生物量、培养基pH值的关系.结果显示不同培养基中白腐菌可去除99%以上的吲哚;吲哚去除率与白腐菌漆酶活率具有较好的相关性,白腐菌漆酶活率达到最高时,可去除97%以上的吲哚;高浓度吲噪会抑制白腐菌的生长,同时也激发了白腐菌漆酶的产生,秸秆滤出液能促进白腐菌的生长和漆酶活力的增长;白腐菌BP最适pH值为6~7,pH值在5~8之间对吲哚都具有较强的降解能力;在HPLC谱图3~6min之间出现的新物质可能有氧化吲哚和靛红.White rot fungus(BP) was selected to degrade indole. Studied were the degradation process and mechanism of indole at two concentration levels in different culture mediums, the relationship between degradation of indole and activity of laccase, the biomass and pH values of culture mediums. The results showed that more 99 % of indole was degraded by white rot fungi in different culture mediums. The degradation rate of indole was correlated closely with the activity of laccase. When the activity of laccase reached maximum, more 97 % of indole was degraded by white rot fungi. Indoles with high concentration could inhibite the growth of white rot fungi and excite the production of laccase. The culture medium of straw leaching liquid could promote the growth of white rot fungi and the activity of laccase. The optimized pH value of white rot fungus(BP) was 6-7. Indole could be degraded effectively when the pH value was between 5-8. The new substances detected on the high performance liquid chromatogram between 3-6 min maybe oxindole and isatin.
分 类 号:X172[环境科学与工程—环境科学]
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