RNA干扰及其在动物传染病预防上的应用  

RNAi and Its Application in Prevention of Animal Infectious Disease

作　　者：段廷云[1] 董振杰[1] 陈红英[1] 袁远[2] 崔保安[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450001 [2]中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨150001

出　　处：《动物医学进展》2006年第5期43-46,共4页Progress In Veterinary Medicine

摘　　要：RNA干扰技术是近几年发展起来的分子生物学新技术,广泛应用于基因功能研究和人畜疾病预防。在RNaseⅢ的协助下,siRNA能够结合并切断相应基因的mRNA,从而阻断基因的表达。siRNA可以是人工体外合成,也可以是转染到细胞内的重组载体转录产生。对碱基上某些基团的取代会影响到siRNA的干扰活性及其对RNA酶的耐受性。由于siRNA只有不到30个碱基的长度,很容易在病毒的不同亚型和变异株之间找到保守序列,且应用后能快速产生抗病毒作用,这是疫苗和抗病毒药物不具备的优点。RNAi is a kind of new molecular biology technique developing in recent years, applicated widespread in the research of gene function, human and animal infectious disease. On the assisting of RNaseⅢ, siRNA can stop the correlated genie expression by binding and shearing out its gene. SiRNA may be artificial or producing by the recombinated vector in cells. Substitution of some groups in the duplo-strand RNA will influence its interfering activity and tolerance to the RNase. Because it is easy relatively to find a conservative sequence less than 30 bp in different subtypes or strains and can activate quickly anti-virus effect, the technique is more potential than vaccine and anti-virus drugs.

关 键 词：RNA干扰 SIRNA 基因沉默 DICER酶 RNA诱导沉默复合物 脱靶 

分 类 号：S852.4[农业科学—基础兽医学] Q522[农业科学—兽医学]