金矿化指示菌的分子生物学鉴定  

Molecular Identification of Gold-Mineralization Indicating Bacteria

作　　者：杨娇艳[1] 张勇[1] 王红梅[2] 宋冬林[3] 程琴[1] 梅娟[1] 吴刚[1] 

机构地区：[1]华中师范大学生命科学学院,湖北武汉430079 [2]中国地质大学环境学院,湖北武汉430074 [3]中国科学院武汉病毒研究所,湖北武汉430071

出　　处：《地球科学（中国地质大学学报）》2006年第3期355-360,共6页Earth Science-Journal of China University of Geosciences

基　　金：国家自然科学基金项目(No.40202011);武汉市晨光计划项目(No.2003500201629);中国地质大学(武汉)环境学院科研类研究项目(04200404).

摘　　要：近年来,土壤微生物蜡样芽孢杆菌(Bacilluscereus)芽孢计数对下伏金矿化的指示作用已被不同国家的地质工作者所证实,但由于鉴别手段的限制,对金矿化指示菌株的鉴定一直停留在传统的微生物分类鉴定上,本研究将分子生物学的技术引入到金矿化指示菌株的鉴定中,从而将微生物找矿中的微生物鉴定手段提高到分子水平.从湖北省嘉鱼县蛇屋山金矿区分离到的矿化指示菌株中随机挑取2株菌Y1和Y2,PCR扩增并测定其16SrDNA和16S-23SrDNA区间序列.经系统发育和生理生化试验综合分析,表明Y1为蕈状芽孢杆菌(B.mycoides),Y2为蜡样芽孢杆菌,两者都属于蜡样芽孢杆菌群,证明蜡样芽孢杆菌群是金矿化指示菌,同时也证明了传统微生物找矿法利用选择性培养基对蜡样芽孢杆菌群菌芽孢计数方法的可靠性和利用分子生物学技术进行采矿的可行性.菌株16SrDNA和16S-23SrDNA区间核苷酸序列的确定也为下一步微生物找矿中的基因探针试剂盒的制作奠定了基础.Recently the microbial mineral exploration method, spore counting of Bacillus cereus in soil samples which is indicative for blind gold ore deposit, has been confirmed by many geologists in different countries. However, the identification of bacterial strains, indicators for gold mineralization, was still done through the culturing method in previous studies. This paper employed molecular biology technique to microbial identification in mineralized areas. Two bacterial strains as indicators of gold mineralization were randomly isolated from the gold deposit of Shewu mountain in Jiayu county, Hubei Province. On the basis of biochemical and physiological identification, phylogenetic analysis dedrived from 16S rDNA and 16S-23S rDNA sequence, strains Y1 and Y2 were identified as Bacillus mycoides and B. cereus respectively, both of which belong to ＂B. cereus group＂. So it is ＂B. cereus group＂ that is the indicator for gold mineralization instead of B. ceresus species. The results assured the reliability of the traditional selective culturing method of B. cereus group, and the feasibility of molecular biology techniques on mineral exploration. In addition, 16S rDNA and 16S-23S rDNA sequence analyses were helpful to make fluorogenic-probe reagent box in future work.

关 键 词：16S RDNA 16S-23S RDNA间区 菌种鉴定 金矿化指示菌 微生物找矿. 

分 类 号：Q93-31[生物学—微生物学]