微囊藻毒素和黄曲霉毒素及伏马菌素联合毒性  

Combinative toxicity of Microcystin,Aflatoxin and Fumonisin

作　　者：熊丽林[1] 唐萌[1] 王加生[2] 曾垂焕[1] 夏婷[1] 王晓娜[1] 

机构地区：[1]东南大学公共卫生学院,南京210009 [2]美国德克萨斯理工大学环境毒理系环境与人类健康研究所

出　　处：《中国公共卫生》2006年第5期548-549,共2页Chinese Journal of Public Health

基　　金：美国癌症研究所/美国国立卫生研究院合作课题项目(1R21CA94683-03)

摘　　要：目的研究微囊藻毒素、黄曲霉毒素、伏马菌素对Hep G2细胞的半数抑制浓度(IC50),探讨3种毒素的联合毒性。方法选用3种毒素各自最具代表性的异构体微囊藻毒素LR、黄曲霉毒素B1和伏马菌素B1,用水溶性四氮唑(WST-1)比色法测定3种物质对Hep G2细胞存活率的影响,计算IC50,并根据等效曲面联合毒性分析模型判断联合作用类型。结果染毒24 h,微囊藻毒素LR对Hep G2细胞的IC50>100μmol/L;黄曲霉毒素B1、伏马菌素B1及3毒素混合物的IC50分别为1.0,399.2和172.0μmol/L。根据等效曲面法求得的相互作用指数α为0.95。结论微囊藻毒素LR、黄曲霉毒素B1和伏马菌素B1对Hep G2的联合毒作用类型为相加作用。Objective To study 50 % inhibition concentration （IC50）of Microcystin, Aflatoxin and Fumonisin and the combinative toxic effect of the three biotoxins in HepG2 cell line. Methods Cell viability and 50 % inhibition concentration （ IC50 ） of Microcystin - LR（ MCLR）, Aflatoxin B1 （AFB1）, Fumonisin B1 （ FB1 ） and the mixture were measured by tetrazolium salt（WST - 1 ）assay with HepG2 cell line. Combinative toxicity of the three blotoxins was evaluated according to response surface analysis. Results Treatment of MCLR for 24 , the ICs0 was more than 100 μmol/L. IC50 of Aflatoxin B1, Fumonisin B1 and the mixture of the three biotoxins were 1.0,399.2 and 172.0 μmoL/L respectively. The interaction index according to response surface analysis was 0.95. Conclusion Combinative toxicity of the mixture falls into the category of additive effect.

关 键 词：联合毒性 细胞毒性 HEP G2细胞 微囊藻毒素 黄曲霉毒素 伏马菌素 

分 类 号：R114[医药卫生—卫生毒理学]