MAPK和MPF对小鼠受精卵有丝分裂期作用  

Study on function of MAPK and MPF in mitosis of mouse fertilized eggs

作　　者：穆润清[1] 贺安宁[1] 王丽[1] 于秉治[2] 

机构地区：[1]中国医科大学附属第一医院检验科,沈阳110001 [2]中国医科大学基础医学院生物化学教研室

出　　处：《中国公共卫生》2006年第5期594-595,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(39730460)

摘　　要：目的探讨有丝分裂促进因子(MPF)和丝裂原活化的蛋白激酶(MAPK)在小鼠受精卵第一有丝分裂期(M期)中的作用及相互关系。方法分别用MAPK激酶特异性抑制剂(U0126)和MPF特异性抑制剂(roscovitine)抑制受精卵细胞MAPK和MPF的活性,通过同位素测定MAPK和MPF的活性变化,利用显微镜进行形态观察来判定MAPK活性变化对受精卵发育的影响。结果在受精卵第一次有丝分裂期,正常组小鼠受精卵MAPK和MPF的活性均有短暂升高。在U0126处理组,MAPK的活性受抑制会导致受精卵处于M期而不发生分裂,但MPF的活性未受到影响。在roscovitine处理组,MPF的活性受到抑制导致受精卵处于M期不发生分裂,此时MAPK不发生活化。结论MAPK和MPF的活化对于小鼠受精卵完成有丝分裂是不可缺少的,在有丝分裂期MPF参与MAPK的活化。Objective To study on the function of mitogen- activated protein kinase（MAPK） and mitosis- promoting factor（MPF）in the first mitosis of mouse fertilized eggs and the relationship between them. Methods Inhibiting the activity of MAPK and MPF by their inhibitor U0126 and roscovitine, we assayed MAPK and MPF activity in the first mitosis and simultaneously detected the influence of the two kinase activity on the early development of fertilized egg by morphology. Results During the first mitosis in normal control group, MAPK and MPF could be activated transiendy. In U0126 group, the activity of MAPK was inhibited by U0126 induced that fertilized eggs could not complete mitosis and stayed in metaphase, but MPF activation had not been influenced. In roscovitine group, the activity of MPF was inhibited by roscovitine caused that fertilized eggs could not complete mitosis and stayed in metaphase, whatever MAPK maintain inactive state. Conclusion MAPK and MPF activation are required for mouse normal mitotic progression. MPF is involved in the activation of MAPK in metaphase.

关 键 词：特异性抑制剂(roscovitine) 有丝分裂促进因子 丝裂原活化的蛋白激酶(MAPK) 有丝分裂 

分 类 号：R114[医药卫生—卫生毒理学]