植酸钠对诱癌大鼠结肠黏膜增殖活性的影响  

INFLUENCE OF INOSITOL HEXAPHOSPHATE ON LARGE INTESTINAL CELL PROLIFERATION ACTIVITY IN CARCINOGEN-TREATED RATS

作　　者：张海平[1] 宋扬[2] 

机构地区：[1]青岛市市立医院营养科,山东青岛266011 [2]青岛大学医学院医学营养研究所

出　　处：《齐鲁医学杂志》2006年第2期116-117,119,共3页Medical Journal of Qilu

摘　　要：目的通过建立大鼠结肠癌动物模型,观察植酸钠对结肠黏膜增殖活性的影响。方法将30只4周龄雄性Wistar大鼠按体质量随机分为植酸组和对照组,植酸组饮水中添加20 g/L植酸钠,对照组自由饮水。两组大鼠同时皮下注射1,2-二甲肼(DMH)诱发结肠癌,观察结肠肿瘤的发生情况并测定结肠黏膜细胞的增殖活性。结果植酸组结肠癌的发生率与对照组比较无显著性差异(P=0.828);但植酸组平均每只鼠的肿瘤个数显著少于对照组(t=3.056,P<0.01);肿瘤体积也显著低于对照组(t=2.291,P<0.05);植酸组结肠黏膜细胞的增殖活性比对照组显著降低(t=2.680,P<0.01)。结论饮水中添加20 g/L植酸钠可降低诱癌大鼠患结肠肿瘤的危险,植酸可能通过降低结肠黏膜细胞的增殖活性发挥其抗肿瘤作用。Objective To evaluate the effect of inositol hexaphosphate （InsP6） on 1, 2-dimethylhydrazine （DMH） induced large intestinal cell proliferation activity in Wistar rats. Methods Thirty four-week old male Wistar rats were randomly divided into two groups, The rats in the treated group were given 20 g/L Na-InsP6 supplemented water, while those in the control group received tap water, The rats were given a weekly injection of DMH to induce large intestinal carcinoma, Total macroscopically visible neoplasms were counted and the volume of individual neoplasmas was calculated. Proliferation cell nuclear antigen （PCNA） of large intestinal was analyzed. Results The incidence of tumors in the treated group was not significantly different from that of the control group （P=0. 828）. However, InsP6 significantly reduced the number of tumor （t=3.056,P〈0.01） and tumor volume （t=2. 291 ,P〈0.05）. Treatment with InsP6 significantly reduced the expression of PCNA（t=2. 680,P〈0.01）. Conclusion Na-InsP6 is effective in reducing the risk of large intestinal cancer in Wistar rats. InsP6 may exert its antineoplastic effect by reducing the expression of PCNA of large intestinal.

关 键 词：肠肿癌 植酸钠 增殖细胞核抗原 

分 类 号：R151.41[医药卫生—营养与食品卫生学]